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A. S. McNEILLY

SUMMARY

Thirty-six series of serial jugular blood samples were collected from 12 goats during suckling during the first 3 weeks of lactation and the transient appearance of oxytocin (5–86 μu./ml plasma) was detected in 24 of the series. Blood oxytocin was assayed on the lactating guinea-pig mammary gland. Forty series of serial blood samples were also collected from 12 goats during hand-milking over the first 6 weeks of lactation and the transient appearance of oxytocin (5–160 μu./ml plasma) was observed in 25 of these.

The results indicated that: (1) oxytocin may be released at any time during the suckling or milking process; (2) in only 16% of experiments where oxytocin was released and 5·6% of all experiments investigated could a conditioned release of oxytocin be induced in goats before suckling and in no case before hand-milking despite a vigorous and prolonged conditioning period; (3) there is a large degree of variability in the pattern of oxytocin release between animals and between individual suckling and milking episodes in the same animal; (4) suckling and hand-milking are equally effective in causing the release of oxytocin; (5) stimuli arising during various stages of the suckling and hand-milking routines may be assigned a figure indicative of the relative effectiveness of these stimuli in terms of percentage probability in causing oxytocin release; (6) there is a greater probability of oxytocin release occurring before suckling than before hand-milking; (7) there is a very marked similarity in the pattern of oxytocin release both during and after teat stimulation in response to suckling and milking stimuli; (8) there is a greater probability of oxytocin being released in response to hand-milking during early lactation than during late lactation; (9) the milk-ejection reflex in the goat does not appear to be important for the achievement of normal milk yields during suckling or hand-milking.

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A. S. McNeilly

Prolactin is the hormone principally involved with stimulating milk production. Therefore, at first sight, a role for prolactin in controlling gonadotrophin secretion may seem strange, especially as its involvement at the ovarian level has only been irrevocably established in rodents, particularly the rat (see McNeilly, 1984). However, in almost all species studied so far, the high levels of prolactin induced by suckling and essential for lactation are also associated with a reduction in normal gonadotrophin secretion, principally the pulsatile secretion of luteinizing hormone (LH), and as a consequence, a suppression of ovarian activity. A similar association between increased levels of prolactin and a reduction in both LH and follicle-stimulating hormone (FSH) occurs during seasonal infertility in male and female ungulates and in pathological hyperprolactinaemia in both men and women.

In view of this apparent relationship between increased prolactin and decreased gonadotrophin secretion, a specific role for prolactin itself in suppressing

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C. HAGEN and A. S. McNEILLY

SUMMARY

Specific radioimmunoassays were used to assess the content of LH, FSH, the gonadotrophin α-subunit and the LH β-subunit in four adult, 19 normal foetal pituitary glands (9·5–32 weeks of gestation) and a pituitary extract from an anencephalic foetus (36 weeks). The hormones and subunits were further identified by column chromatography on Sephadex G-100. All pituitary glands contained free α-subunit and intact LH but the α-subunit:LH ratio was significantly higher in the early foetal pituitaries (9·5–16 weeks) than in the four adult pituitaries. Only small or undetectable amounts of LH β-subunit and 'undetectable' FSH were found in these early foetal pituitaries (9·5–11·5 weeks). The concentration of intact hormones or subunits in the pituitaries showed no significant sex difference in any of the groups. In contrast to these results, only α-subunit was detectable in the pituitary of the anencephalic foetus.

For 14 early foetuses (age of gestation 10–16 weeks) the serum levels of LH–HCG, FSH, and α-subunit in the circulation were significantly higher than in 26 foetuses at term (37–41 weeks). On the basis of these results a theory for the development of the gonadotrophin secretion from the foetal pituitary gland is outlined.

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A. S. McNEILLY and P. ANDREWS

SUMMARY

Prolactin was isolated from frozen goat pituitary glands by a simple procedure involving gel filtration and chromatography on DEAE-cellulose. The major product (yield, 2·5 mg/g pituitary tissue) had high pigeon crop sac-stimulating activity (27 i.u./mg) and was free of growth hormone and other pituitary hormones. The molecular weight was similar to that of ovine prolactin.

Caprine prolactin was immunologically indistinguishable from ovine prolactin in radioimmunoassays, in which ovine prolactin antiserum and either ovine or caprine prolactin labelled with 125I were used.

The results indicate that caprine and ovine prolactin are closely related and that radioimmunoassay for ovine prolactin may be used to estimate caprine prolactin in serum.

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J Brooks and A S McNeilly

Abstract

To investigate the regulation of the sheep gonadotrophin-releasing hormone receptor (GnRH-R) gene expression, two different treatment regimes were used. Experiment 1 examined the effects of twice daily injections of ovine follicular fluid (oFF, 15 ml s.c.) as a source of inhibin, and daily GnRH antagonist injections (Nal-Glu.HOAc, 2 mg s.c.) on days 9–12 of the oestrous cycle. Luteolysis was induced on day 12 with prostaglandin (PG) and the ewes killed at two different stages; day 12 (luteal) and 18 h after PG injection. Experiment 2 examined the effect of a single injection of oestradiol benzoate (100 μg i.m.) 18 h before death in luteal phase ewes and ewes chronically implanted with the GnRH agonist, buserelin. In both experiments, pituitaries were removed at death for determination of pituitary GnRH binding, LH content and levels of GnRH-R and LHβ mRNA. In addition in experiment 1, follicles ≥2·5 mm were dissected from the ovaries for determination of oestradiol content.

In experiment 1, oFF treatment during the luteal phase completely inhibited follicle oestradiol production but was without effect on the other parameters measured. After cessation of oFF treatment and induction of luteolysis, a significant (P<0·05) increase in plasma LH occurred but the normal follicular increase in both GnRH-R mRNA levels and GnRH binding seen in control ewes was prevented. GnRH antagonist treatment alone or in combination with oFF also inhibited follicle oestradiol production, prevented the increase in GnRH-R mRNA, completely inhibited GnRH binding and significantly decreased LHβ mRNA levels. Pituitary LH content was unaffected by any treatment. In experiment 2, oestradiol treatment did not affect GnRH-R mRNA levels, GnRH binding, LHβ mRNA or pituitary LH content in luteal phase ewes, whilst chronic GnRH agonist treatment acted to decrease these parameters dramatically. A single injection of oestradiol in the GnRH agonist treated ewes significantly (P<0·05) increased GnRH-R mRNA levels and completely restored GnRH binding to luteal levels, without any effect on LHβ mRNA or pituitary LH content.

These results suggest that the control of GnRH receptor expression in the sheep is directly related to oestradiol and not to the action of GnRH itself.

Journal of Endocrinology (1994) 143, 175–182

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G. A. Lincoln and A. S. McNeilly

ABSTRACT

Changes in the concentration of inhibin, FSH, LH and testosterone were measured in the peripheral blood of adult Soay rams during a reproductive cycle induced by exposure to an artificial lighting regimen (long days with a 16-week period of short days) or treatment with melatonin (long days with a 12-week period when melatonin was administered daily in mid-light phase to simulate the effect of short days). In both experimental situations, changes in the plasma concentrations of inhibin occurred in parallel with the cycle in the diameter of the testes with a four- to fivefold increase in the inhibin concentrations from the nadir to the peak of the testicular cycle. Increases in the plasma concentrations of FSH, LH and testosterone also occurred in association with the reactivation of the reproductive axis. The weekly changes in the plasma concentrations of inhibin were positively correlated with the changes in plasma FSH values during the developing and regressing stages of the testicular cycle but negatively correlated during the active stage.

In a group of castrated rams exposed to the same lighting regimen, the plasma concentrations of inhibin were always below the detection limit of the radioimmunoassay. The testosterone values were also very low in the castrates while the plasma concentrations of FSH and LH were 10-50 fold higher than normal and varied in relation to the light cycle.

The results show for the first time that inhibin is secreted into the peripheral blood in the ram exclusively from the testes. The positive correlation between the changes in plasma concentrations of FSH and inhibin during the developing and regressing phases of the testicular cycle indicate that FSH stimulates the secretion of inhibin. The negative correlation between FSH and inhibin in the active phase of the testicular cycle, is consistent with the role of inhibin in the negative-feedback control of FSH secretion. This is only evident because the testes undergoes reactivation of its full function during the change from the regressed to the active state which is especially obvious in the highly seasonal Soay ram.

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A. S. McNEILLY and C. A. FOX

Although certain of the prostaglandins are known to be potent smooth-muscle contracting agents (Horton, 1969), very little is known of their action on the mammary gland. Prostaglandin F2α (PGF2α) had no effect on the mammary epithelium of the mouse or on blood flow through the perfused goat udder (J. L. Linzell, personal communication; cited by Pickles, 1967). PGF1 had no effect per se on the milk-ejection pressure when given by close i.a. or i.v. injection in the lactating rabbit (Türker & Kiran, 1969) but did decrease the milk-ejection activity of oxytocin in the rabbit (Türker & Kiran, 1969) and abolished the milk-ejection activity of oxytocin in the rat (Haldar, Maiweg & Grosvenor, 1970).

We now report results obtained during investigations into the effect of prostaglandins in the lactating guinea-pig assay for oxytocin (Tindal & Yokoyama, 1962). Changes in intramammary pressure were recorded after the close

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R J W Currie and A S McNeilly

Abstract

Changes in LH secretory granules in pituitary gonadotrophs throughout the sheep oestrous cycle were determined by immunogold localisation of LH at ultrastructural level by electron microscopy. Oestrous cycles in Welsh Mountain ewes were initially synchronised with progestagen sponges and studies carried out in the subsequent cycle. Animals were allocated at random to six groups each of five animals, one killed on day 12 of the luteal phase and the other groups after prostaglandin (PG)-induced luteal regression at PG plus 18 h (early follicular phase), oestrus (PG plus 33·6±1·0 h), oestrus plus 9 h just before the preovulatory LH surge, 1 h after GnRH agonist-induced LH surge at PG plus 48 h (mid-LH surge) and oestrus plus 24 h, after the preovulatory LH surge. Blood samples collected throughout confirmed the pulsatile secretion of LH before and the timing in relation to the preovulatory LH surge. Pituitaries were dissected and processed for transmission electron microscopy and frozen for later extraction of mRNA. Only a single type of LH cell was present in the sheep pituitary. In the luteal phase, LHimmunopositive secretory granules were distributed throughout the cytoplasm in 80% of cells while in 20% of cells granules were polarised to the region of the cell next to a vascular sinusoid. The percentage of polarised cells increased during the follicular phase to 45% at oestrus, 75% at oestrus plus 9 h just before the LH surge and 90% in mid-LH surge. Cell size increased in parallel with polarisation. Gonadotrophs after the LH surge were almost totally devoid of LH granules but prominent LHβ immunoreactivity was observed in the rough endoplasmic reticulum. Analysis of granule diameters revealed a single class of granules with a maximum diameter of 300 nm. Polarised cells had significantly fewer 130–150 nm granules than non-polarised cells, suggesting preferential exocytosis of LH-containing granules of this size from polarised cells. Northern analysis showed that LHβ mRNA levels decreased from luteal through the follicular phase. These results suggest that the preovulatory LH surge in sheep is not related to a change in synthesis of LH but to a progressive recruitment of gonadotrophs into a releasing state, priming, as indicated by polarisation of secretory granules to the region of the cell next to the vascular system.

Journal of Endocrinology (1995) 147, 259–270

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J. M. Wallace and A. S. McNeilly

ABSTRACT

Treatment of Damline ewes with twice-daily i.v. injections of bovine follicular fluid during the luteal phase for 10 or 2 days before prostaglandin-induced luteolysis resulted in a delay in the onset of oestrous behaviour and a marginal increase in ovulation rate. During the treatment cycle, blood samples were withdrawn at 15-min intervals for 25 h from 08.00 h on days 1, 6 and 10 (day 0 = oestrus). At all three stages of the luteal phase, plasma FSH concentrations were suppressed relative to controls 3 h after the 09.00 h injection of follicular fluid and remained low until

06.00 h on the following day. In the 10-day treatment group LH pulse amplitude was significantly greater than that of controls on days 6 and 10. Pulse frequency remained high throughout treatment and was significantly higher relative to controls on day 10 despite normal progesterone levels. The results suggest that the higher pulsatile LH secretion during the luteal phase is due to reduced negative feedback effects of oestradiol occurring as a result of the follicular fluid-induced reduction in FSH.

J. Endocr. (1986) 111, 317–327

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A. S. McNeilly and D. W. Lincoln

To investigate the role of the pineal gland in the long-term suppression of gonadotrophin secretion induced by prolactin, the effects of pinealectomy were studied in adult male rats with hyperprolactinaemia produced by the transplantation of two pituitary glands under the kidney capsule.

Pinealectomy had no effect on basal levels of LH, FSH or prolactin. The presence of pituitary transplants induced a significant twofold increase in prolactin levels and a prolonged suppression in both LH and FSH. These changes were not affected by pinealectomy. Castration resulted in a similar rise in plasma levels of LH and FSH in rats with and without pituitary transplants. In control rats this rise in LH and FSH was reduced by testosterone-containing silicone elastomer implants (s.c) of 10 mm in length and delayed by implants of 30 mm. These rises in LH and FSH were significantly delayed (10-mm implant) or abolished (30-mm implant) in rats with pituitary transplants indicating an increase in sensitivity of the hypothalamic-pituitary axis to the negative feedback effects of testosterone in these animals compared to controls. These responses were not affected by pinealectomy.

These results suggest that the pineal gland is not involved in the mechanism whereby pituitary grafts, possibly through their secretion of prolactin, cause long-term suppression of gonadotrophin secretion.