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Using tritiated oestradiol of very high specific activity and sensitive counting techniques, Jensen & Jacobson (1962) were able to investigate the distribution of this hormone among target and other organs after the injection of physiological doses into Sprague—Dawley rats. It is also possible to demonstrate the uptake of labelled oestradiol and its distribution within the tissues by the conventional autoradiographic stripping film technique after incubation of the tissues in very low concentrations of the hormone.
Virgin female Sprague—Dawley rats, approximately 12 weeks old of a mean weight of 160 g., were used. They were killed by dislocation of the neck, and small cylinders (2–3 mm. in length) of the uterine horns and of small intestine, and also small pieces of liver (maximum thickness 3 mm.), were placed in tissue culture medium '199' containing (6,7-3H)oestradiol-17β (specific activity 150 mc/mg.; New England Nuclear Corporation). The hormone was added
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SUMMARY
The uptake of injected [6,7-3H]oestradiol by 9,10-dimethyl-1,2-benzanthracene-induced mammary tumours of the rat was investigated. Hormone-responsive adenomata differed from unresponsive adenomata in their ability to concentrate the hormone. The concentration of tritiated oestrogen present as free steroid in adenomata which failed to regress after ovariectomy was twice that found in muscle 20 min. after injection, and this ratio did not change significantly for 2 hr. after injection. Hormone-responsive adenomata concentrated the hormone to a much greater extent: the amount of tritiated oestrogen/mg. wet weight continued to rise for 100 min. after injection, when the concentration of radioactive steroid present in the tumours was 8–20 times that present in muscle. In this respect, the hormone-responsive tumours behaved in a similar way to the uterus and other target organs for oestrogen which have been investigated by other authors. Mixed adenomata, that is, those which contained both hormone-responsive and -unresponsive tissue, and fibroadenomata concentrated the injected oestradiol to an extent intermediate between the wholly responsive and wholly unresponsive adenomata.
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SUMMARY
The weight of the accessory sex glands, and the citrate and nucleic acid content of the ventral prostate (VP) glands, were examined in animals which had been adrenalectomized 30 days previously and in intact control rats. Adrenalectomy greatly reduced the citrate content, but did not affect the nucleic acid content of the VP. It was concluded that the adrenals stimulated prostatic function rather than growth.
The concentration of radioactivity in the accessory sex glands of adrenalectomized and/or castrated animals after the injection of [3H]testosterone was compared with that in sham-operated controls. Radioactivity was related to the DNA content of the VP of the four groups. It was concluded that adrenalectomy facilitated the uptake and/or retention of androgen.
[3H]Corticosterone was not retained by any of the accessory sex glands of animals adrenalectomized and castrated 2 days previously. It seems unlikely that the glands should be considered 'target organs' for corticosterone in the accepted sense of the term, but it is suggested that corticosteroids may help to maintain the balance between differentiation and growth in the VP by influencing the metabolism of androgens by the prostate gland.