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Search for other papers by R. G. GOSDEN in
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Recently a single hypothalamic hormone, termed luteinizing hormone releasing hormone (LH-RH), capable of releasing luteinizing hormone (LH) and follicle-stimulating hormone (FSH) from the pituitary has been chemically characterized and synthesized (Matsuo, Arimura, Nair & Schally, 1971; Matsuo, Baba, Nair, Arimura & Schally, 1971) and has been shown to be biologically active in several species (Schally, Arimura & Kastin, 1973). Initial attempts to demonstrate LH-RH action in 4-day cyclic mice treated with sodium barbitone in pro-oestrus were unsuccessful because barbitone did not consistently block ovulation (R. G. Gosden, unpublished results). In this report we demonstrate the action of LH-RH in adult female mice as shown by the induction of ovulation and the increase of serum LH.
Young virgin (8–14 weeks) CFLP mice (Carworth Europe) were injected with 3 i.u. pregnant mare serum gonadotrophin (Intervet) i.p. at 16.00–17.00 h without reference to the stage of the oestrous cycle. Approximately 44 h later
Search for other papers by CAROL READHEAD in
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SUMMARY
An immunoradiometric assay for human follicle-stimulating hormone (FSH) is described. A non-specific antiserum to human FSH was purified by incubation of the antiserum with human luteinizing hormone and thyroid-stimulating hormone before its reaction with an immunoadsorbent containing human FSH. The specific antibodies to human FSH isolated on immunoadsorbent were iodinated and used for the FSH assay. The sensitivity of the assay was improved by the extraction of plasma FSH onto antibody-coated polyethylene tubes before its reaction with iodinated FSH antibodies producing a two-site assay. Optimal conditions for the preparation and use of antibody-coated tubes in such a procedure are described. Using serial extraction a sensitivity of 11 pg/ml (0·1 mu./ml) was achieved. There was good agreement between the results obtained with this method and those obtained with radioimmunoassay. The advantages of the increased sensitivity of this new method are discussed.
