Search Results
You are looking at 1 - 1 of 1 items for
- Author: Caroline Rönnlund x
- Refine by access: All content x
Search for other papers by Patrick Müller in
Google Scholar
PubMed
Search for other papers by Kenneth W Merrell in
Google Scholar
PubMed
Search for other papers by Justin D Crofts in
Google Scholar
PubMed
Search for other papers by Caroline Rönnlund in
Google Scholar
PubMed
Search for other papers by Chin-Yo Lin in
Google Scholar
PubMed
Search for other papers by Jan-Åke Gustafsson in
Google Scholar
PubMed
Search for other papers by Anders Ström in
Google Scholar
PubMed
Regulation of hairy and enhancer of split homologue-1 (HES-1) by estradiol and all-trans retinoic acid affects proliferation of human breast cancer cells. Here, we identify and characterize cis-regulatory elements involved in HES-1 regulation. In the distal 5′ promoter of the HES-1 gene, we found a retinoic acid response element and in the distal 3′ region, an estrogen receptor α(ER)α binding site. The ERα binding site, composed of an estrogen response element (ERE) and an ERE half-site, is important for both ERα binding and transcriptional regulation. Chromatin immunoprecipitation assays revealed that ERα is recruited to the ERE and associates with the HES-1 promoter. We also show recruitment of nuclear receptor co-regulators to the ERE in response to estradiol, followed by a decrease in histone acetylation and RNA polymerase II docking in the HES-1 promoter region. Our findings are consistent with a novel type of repressive estrogen response element in the distal 3′ region of the HES-1 gene.
