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D. R. INMAN
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R. E. W. BANFIELD
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R. J. B. KING
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SUMMARY

The intracellular localization of [6,7-3H]oestradiol in rat pituitary, liver and uterus has been studied using autoradiography. In the anterior pituitary the oestrogen has been located on the nuclear membrane and nucleolus of the eosinophils. The posterior pituitary did not produce positive autoradiographs and only occasional silver grains were seen in the pars intermedia. In the liver the silver grains were uniformly distributed throughout the cytoplasm; only very occasionally were grains associated with the nucleus. No satisfactory results were obtained with the uterus. Labelled erythrocytes were seen in all the tissues studied. The results are discussed in relation to the mode of action of oestradiol.

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R. J. B. KING
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J. GORDON
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DOROTHEA M. COWAN
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D. R. INMAN
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SUMMARY

The intranuclear localization of [6,7-3H]oestradiol-17β (oestradiol) has been studied in nuclei from dimethylbenzanthracene (DMBA)-induced rat mammary adenocarcinoma, anterior pituitary, uterine epithelium and kidney. The evidence suggests that, with the possible exception of uterine epithelium, most of the oestrogen is associated with an acid-labile nonhistone component of the chromatin. No evidence has been found to suggest that oestradiol is associated with any particular type of chromatin. These results are compatible with the view that oestradiol affects cellular function in some tissues by altering the priming ability of the chromatin DNA.

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