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H Kayed, J Kleeff, S Keleg, MW Buchler, and H Friess

Indian hedgehog (IHH) and its receptors patched (PTC) and smoothened (SMO) belong to the hedgehog family of signaling molecules, which are essential for a variety of patterning events during mammalian tIssue development. IHH plays a role in pancreas organogenesis and differentiation, as well as in the regulation of insulin production. In the present study, the expression of IHH and its receptors was analyzed in normal human pancreatic and chronic pancreatitis (CP) tIssues using Northern blotting, immunohistochemistry and Western blotting, and was correlated with clinicopathological parameters. In addition, the effects of inhibition and stimulation of the hedgehog signaling pathway on cell growth were determined in TAKA-1 normal pancreatic ductal cells. IHH mRNA was expressed in the normal human pancreas and CP tIssues, with slightly higher expression levels in CP. Using immunohistochemistry, IHH and its receptors were localized mainly in the islet cells of the normal pancreas. In CP, IHH and its receptors were present in the cells forming tubular complexes and in the islets with a different signal pattern compared with the islets in the normal pancreas. Correlation between diabetic and non-diabetic CP patients revealed no significant difference in IHH, SMO, or PTC immunoreactivity. Inhibition of hedgehog signaling in TAKA-1 pancreatic ductal cells using cyclopamine significantly reduced their growth through cell cycle arrest, while stimulation of the IHH pathway enhanced the growth of these cells. In conclusion, IHH and its receptors are expressed in the normal human pancreas and in CP, yet with a different distribution and cellular localization. IHH signaling may be involved in the pathogenesis of CP, i.e. in the formation and proliferation of tubular complexes and in islet cell dysfunction.

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Department of Biodynamics, Weizmann Institute of Science, Rehovot, Israel

(Received 4 June 1974)

Luteinizing hormone (LH) stimulates ovarian ornithine decarboxylase (ODC), the first enzyme in the pathway of polyamine synthesis, when injected into mature (Kobayashi, Kupelian & Maudsley, 1971; Jänne & Williams-Ashman, 1971) or immature rats (Kaye, Icekson, Lamprecht, Gruss, Tsafriri & Lindner, 1973). Kobayashi et al. (1971) observed a rise in ovarian ODC activity on the afternoon of prooestrus, which may be related to the ovulatory process (Kobayashi et al. 1971) and/ or the early stages of corpus luteum formation (Williams-Ashman, Jänne, Coppoc, Geroch & Schenone, 1972). We show here that ovarian ODC activity is stimulated by LH preferentially in the Graafian follicle.

Groups of three to five Wistar-derived female rats (approximately 100 days old) selected after at least two successive regular 4-day oestrous cycles, were used for surgical isolation of Graafian follicles and corpora lutea. Only corpora lutea