Search Results
You are looking at 1 - 1 of 1 items for
- Author: J. A. Foulkes x
- Refine by access: All content x
Search for other papers by D. J. Groves in
Google Scholar
PubMed
Search for other papers by M. J. Sauer in
Google Scholar
PubMed
Search for other papers by P. Rayment in
Google Scholar
PubMed
Search for other papers by J. A. Foulkes in
Google Scholar
PubMed
Search for other papers by B. A. Morris in
Google Scholar
PubMed
ABSTRACT
Peripheral blood mononuclear cells were collected from a sheep immunized against progesterone-11α-hemisuccinate-ovalbumin. Following fusion with NS1 mouse myeloma or heteromyeloma cells, a large number of hybrid colonies was established. These were screened for the production of sheep antibodies to progesterone. Twenty-four cell lines were cloned and one was stabilized. This cell line, O/MP.1A9.D7B2, produced a high-affinity ovine immunoglobulin G1 (dissociation constant 4·8 pmol/l) with a high degree of specificity for progesterone. The antibody was substituted into a competitive enzyme-linked immunosorbant assay for the measurement of progesterone in bovine milk, originally established using an ovine polyclonal antibody, and the results were compared. The monoclonal antibody produced an assay with a lower limit of detection and a greater degree of discrimination than the polyclonal antiserum.
Journal of Endocrinology (1990) 126, 217–222
