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J. Balthazart and M. Schumacher

ABSTRACT

Testosterone metabolism in the brain and pituitary and cloacal glands of male and female Japanese quail was studied in vitro during sexual maturation (from 1 day to 5 weeks after hatching).

The production of 5α-dihydrotestosterone in the hyperstriatum and cloacal gland and that of androstenedione in the cloacal gland of males was highest at 1 day after hatching, which could be related to the peak of plasma androgens previously demonstrated in neonatal quail. 5β-Reductase activity was very high in the brain, but not the pituitary or cloacal glands of young chicks and decreased markedly, especially in the hypothalamus, during sexual maturation. As 5β-reduced metabolites of testosterone are inactive androgens, it is suggested that the decrease of 5β-reductase activity with age corresponds to a potentiation of the effects of testosterone at the level of the brain.

J. Endocr. (1984) 100, 13–18

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C. Alexandre and J. Balthazart

ABSTRACT

The effects of antioestrogens, antiandrogens and of various inhibitors of testosterone metabolism on testosterone metabolism in the quail hypothalamus and cloacal gland were studied by an in-vitro radio-enzymatic assay. It was found that antioestrogens and antiandrogens generally had little or no effect on aromatase and 5α- and 5β-reductases of testosterone, except when used at very high doses. The 5α-reductase inhibitor, 17β-N,N-diethylcarbamoyl-4-methyl-4-aza-5α-androstan-3-one, inhibited both 5α- and 5β-dihydrotestosterone production without markedly affecting aromatase activity. Surprisingly, the aromatase inhibitor, 1,4,6-androstatriene-3,17-dione, inhibited not only the production of oestradiol but also that of 5β-dihydrotestosterone and, to a lesser extent, 5α-dihydrotestosterone. These unexpected properties should be taken into account when interpreting the results of in-vivo experiments using these compounds.

J. Endocr. (1987) 112, 189–195

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J. BALTHAZART and J. C. HENDRICK

Laboratoire de Biochimie et Laboratoire de Radioimmunologie, Université de Liège, 17 Place Delcour, B-4020 Liège, Belgium

(Received 31 October 1977)

It is well known that the concentration of luteinizing hormone (LH) in the plasma is higher in male than in female chickens (Sharp, 1975) and quail (Nicholls, Scanes & Follett, 1973), probably as a result of greater steroid feedback in the female birds (Davies, 1976). Guichard, Cédard, Mignot, Scheib & Haffen (1977) have shown that embryonic female chick gonads secrete at least ten times more oestradiol than gonads from embryonic male chicks.

We have therefore investigated the relative effectiveness of androgens and oestrogens in the control of the level of LH in the male chick and whether non-aromatizable 5α-reduced androgens could depress this level, as has recently been shown in the adult quail (Massa, Davies & James, 1978).

Nine groups of between three and seven 1-day-old male chicks were injected

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J. Balthazart and M. A. Ottinger

ABSTRACT

Testosterone metabolism was studied by an in-vitro technique in the brain and cloacal gland of young male and female quail at different ages ranging from 7 days of incubation to 2 days after hatching. Very active metabolism, leading almost exclusively to the production of 5β-reduced compounds, was observed. 5β-Reductase activity remained high throughout the incubation period in the hypothalamus, decreased around the time of hatching in the cerebellum and decreased progressively between days 7 and 15 of incubation in the cloacal gland. These changes could be involved in the control of sexual differentiation: the high 5β-reductase in the brain possibly protects males from being behaviourally demasculinized by their endogenous testosterone while the decreasing 5β-reductase in the cloacal gland would progressively permit the masculinization of that structure.

J. Endocr. (1984) 102, 77–81

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M. Schumacher, J. Sulon and J. Balthazart

ABSTRACT

Serum concentrations of testosterone, 5α-dihydrotestosterone, oestradiol and progesterone were measured by radioimmunoassay combined with Celite chromatography in male and female Japanese quail (Coturnix coturnix japonica) during the second half of embryonic life (days 9–17 of incubation) and during the first 5 weeks after hatching. The mean level of each of the four steroids was significantly affected by the age of the birds. An overall effect of sex was detected by analysis of variance only on oestradiol concentrations, with females having higher serum concentrations than males during most of the age range studied. Significant peaks of testosterone and progesterone were also detected around hatching time. These results are consistent with the view that oestradiol is the major hormone implicated in the sexual differentiation of reproductive behaviour in the quail. The relationships between the circulating concentrations of oestradiol during ontogeny and the critical period of differentiation as postulated by currently accepted models is also discussed.

J. Endocr. (1988) 118, 127–134

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J. Balthazart, M. Schumacher and G. Malacarne

ABSTRACT

It has been suggested that testosterone is less effective at inducing crowing behaviour in young birds than in adults because of the presence of higher levels of steroid 5β-reductase in the young brain, which converts testosterone to inactive 5β-reduced metabolites. This hypothesis was tested indirectly by comparing the relative potencies of 5α-dihydrotestosterone (5α-DHT), which cannot be converted to 5β-metabolities, and testosterone at inducing crowing in young gonadectomized male and female quail. The promotion of cloacal gland growth by these treatments was also assessed since there are no age-related changes in 5β-reductase in this organ.

Silicone elastomer implants (2·5, 5 and 10 mm) containing 5α-DHT were more effective at stimulating crowing than similar implants of testosterone whilst there was little difference in their potency at inducing cloacal gland growth. These results are consistent with the hypothesis that brain steroid 5β-reductase regulates the behavioural activity of testosterone in the brain of young birds.

J. Endocr. (1984) 100, 19–23

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J. BALTHAZART, J. D. BLAUSTEIN, M. F. CHENG and H. H. FEDER

A cytoplasmic progestin receptor has been characterized in the brain of castrated ring doves using an in-vitro assay that measures the binding of a synthetic progestin, [3H]17α,21-dimethyl-19-nor-pregna-4,9-diene-3,20-dione(promegestone; R5020). The affinity of the receptor was similar in both the hyperstriatum and the hypothalamus (K d≃4 × 10−10 mol/l). Its concentration was higher in the anterior hypothalamus–preoptic area (63 ± 4 fmol/mg (s.e.m.) protein) than in other brain regions (posterior hypothalamus, 33 ± 5; hyperstriatum, 28 ± 3; midbrain, 17 ± 4 fmol/mg protein; n = 7). Progesterone and R5020 competed well for binding but oestradiol and 5β-dihydrotestosterone did not. Corticosterone and, to a lesser extent, testosterone and 5α-dihydrotestosterone competed for binding but much higher concentrations were required than for progestins. Injections of testosterone (200 pg testosterone propionate daily for 7 days) significantly increased the concentration of progestin receptors in the anterior and posterior hypothalamus without having any significant effect on other brain areas. Shorter treatment, lasting for 2 days, with testosterone propionate (200 μg daily), 5α-dihydrotestosterone (200 μg daily) or oestradiol benzoate (50 μg daily) did not always cause this increase but seven injections of oestradiol benzoate (50 pg daily for 7 days) were even more effective than seven injections of testosterone propionate (200 μg daily for 7 days). These data suggested that the sensitivity to progesterone of the brain of the bird changes as a consequence of increases in the level of testosterone in the circulation.