Search Results
You are looking at 1 - 1 of 1 items for
- Author: J. C. MACOME x
- Refine by access: All content x
Search for other papers by D. N. DE CARLI in
Google Scholar
PubMed
Search for other papers by A. CASTRO-VAZQUEZ in
Google Scholar
PubMed
Search for other papers by J. C. MACOME in
Google Scholar
PubMed
Search for other papers by J. M. ROSNER in
Google Scholar
PubMed
Several authors have proposed histamine as the mediator of oestrogen action (Holden, 1939; Spaziani & Szego, 1958), and the uterine release of histamine during early pregnancy has also been considered an oestrogenic effect (Shelesnyak, 1960), though later work proved this to be unlikely (Marcus & Shelesnyak, 1967). We have studied the influence of histamine on the uptake of oestradiol-17β by its target organs.
Adult female Wistar rats, ovariectomized 2 weeks previously, were injected s.c. with 5·76 × 106 d.p.m. of [6,7-3H]oestradiol-17β in 0·5 ml 0·9% NaCl solution. Oestradiol-17β (Nuclear Chicago Inc., sp. act. 40 Ci/mm) was previously purified by paper chromatography in benzene—formamide (Neher, 1964). Immediately after injection of the steroid, the experimental group received 50 mg histamine dihydrochloride s.c. in 0·5 ml 0·9% NaCl solution while control animals received only the vehicle.
The animals were killed 75 min later by cervical dislocation, and radioactivity was
