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M. A. AL-AWQATI
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R. J. ETHERIDGE
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J. G. GRUDZINSKAS
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T. CHARD
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A radioimmunoassay for a new human adrenal specific protein (ASP) is described. The assay has a lower limit of detection of approximately 50 μg/l. The ASP extracted and purified from adrenal glands was used for standardization, radio-iodination and immunization of rabbits. Adrenal specific protein can be detected in normal human serum and plasma (240 ± 80 and 190 ± 60 μg/ml (means ± s.d.) respectively), tissue levels were found in extracts of adrenal medulla, with appreciable amounts in the adrenal cortex, pituitary gland, seminal vesicle and testis, and low levels in all other tissues examined. The highest concentrations in biological fluids were found in seminal plasma and to a lesser extent in milk and menstrual fluid.

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R. J. L. HOOPER
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R. E. SILMAN
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R. M. LEONE
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M. D. A. FINNIE
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S. J. CARTER
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J. G. GRUDZINSKAS
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Y. B. GORDON
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DIANE T. HOLLAND
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T. CHARD
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P. E. MULLEN
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I. SMITH
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SUMMARY

The pineal indole 5-methoxytryptophol (ML) has been shown to have an antigonadal activity when administered to experimental animals, but data on its normal pattern of secretion have been lacking. Using a new gas chromatography–mass spectrometry assay, the concentration of ML at various phases of the human menstrual cycle has been studied. Daily samples were obtained throughout the month from five women with a normal cycle and two women taking an oral contraceptive. In women with a normal cycle levels of ML were found to be significantly lower in the last third of their cycle; this change was not seen in women taking an oral contraceptive who had low levels throughout the month. The changes in concentration of ML did not correlate with the changes in concentration of gonadotrophins.

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