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SUMMARY
Antisera were produced in rabbits against highly purified preparations of human LH (2000 or 10000 i.u./mg), human FSH (5500 i.u./mg), and human TSH (7·5 i.u./mg). Most rabbits produced antisera of high titre and high avidity. Cross-reactions were minimal between human TSH and human chorionic gonadotrophin (HCG) and between human FSH and HCG but marked between human LH and HCG. TSH and FSH also showed a constant but relatively weak cross-reaction. LH cross-reacted with FSH to a higher degree than did HCG. The avidity of the antisera was high. It was concluded that much of the lack of specificity recorded for glycoprotein antisera are effects of impure immunogens. Some of the true cross-reactions are probably explained by shared antigenic determinants of the β-subunits. Unadsorbed antisera could be used for assay of FSH and TSH in plasma from pregnant women.
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Search for other papers by J. MULDER in
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SUMMARY
The concentrations of luteinizing hormone releasing hormone (LH-RH) and luteinizing hormone (LH) were determined by radioimmunoassay in blood samples taken at intervals after the administration of different doses of synthetic LH-RH administered either intravenously or intranasally in healthy fertile men. Intranasal administration of LH-RH caused a dose-dependent increase of plasma LH with the peak occurring later than that after intravenous injection. The intravenous route was approximately 100 times more effective than the intranasal route in terms of the dose of LH-RH necessary to achieve an LH response of similar magnitude, but the route through the nasal mucosa seems a safe and convenient way for LH-RH administration. The characteristics of the disappearance curve, metabolic clearance rate and volumes of distribution of LH-RH in man are compared with those found by others.
