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- Author: J. d'A. JEFFERY x
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SUMMARY
The metabolic fate of administered progesterone (pregn-4-en-3,20-dione) has been investigated in three pig-tail monkeys (Macaca nemestrina).
Androsterone (3α-hydroxy-5α-androstan-17-one) was a quantitatively important urinary metabolite. Metabolites were also found in substantial amounts in fractions corresponding to pregnanediol and to more polar compounds, but 5β-pregnane-3α,20α-diol itself was not a major urinary metabolite.
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Urine was collected from a female pig-tail monkey (Macaca nemestrina) receiving intramuscular injections of [4-14C]progesterone (0·234μc, sp. act. 11·7 mμc/mg. in 1·0 ml. arachis oil) twice daily for 5 days. A fraction more polar than 5β-pregnane-3α,20α-diol was prepared by acid hydrolysis and chromatography on alumina (Jeffery, 1966). It was rechromatographed and a fraction containing 6-oxygenated metabolites of progesterone (James & Fotherby, 1965) was collected. On subsequent chromatograms, at least 70 % of the radioactive material in this fraction was found in a small region, regarded as the position of the main metabolite or its derivative. Paper chromatography and thin-layer chromatography (Table 1) showed that this metabolite behaved chromatographically like 3α,6α-dihydroxy-5β-pregnan-20-one.
An aliquot (1670 disintegrations/min.) of the alumina column fraction was purified by thin-layer chromatography on silica gel GF (ethyl acetate: acetone, 4:1, v/v). Material eluted from the zone corresponding to 3α,6α-dihydroxy-5β-pregnan-20-one was chromatographed on paper (benzene: methanol: water, 10:7:3,
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ABSTRACT
Rat liver exhibits a reversed sexual dimorphism of its two endogenous soluble carbonic anhydrase (CA) isozymes, CA II and CA III. Normal males have hepatic CA III concentrations ten–twenty times those in the female, while female liver contains two–three times more CA II than the male. Hypophysectomy abolishes this sexual differentiation, having no effect on male liver but producing isozyme concentrations in the female liver similar to those in the male. Infusion of a continuous level of GH into male rats induces a female-like isozyme pattern for both CA II and CA III.
J. Endocr. (1986) 110, 123–126
