The metabolism of 11-deoxycorticosterone (DOC) in vitro by homogenates of adrenal glands from various species has been studied.
Homogenates of 'non-fatty' adrenal glands, especially those prepared from golden hamster adrenals, were shown to possess an active 19-hydroxylating enzyme system. In the case of the golden hamster adrenal, approximately 30% of the added DOC was converted to 19-hydroxy-11-deoxycorticosterone (19-OH-DOC). Negligible transformation was observed using homogenates prepared from 'fatty' adrenal glands, similar to the type found in man. 19-OH-DOC was, however, formed from DOC by a homogenate of a human foetal adrenal gland.
No relationship was observed between the 11β-hydroxylating activity of the adrenal homogenate, and the reported cortisol/corticosterone ratio found in the adrenal venous blood of various animals.