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- Author: K. E. KIRKHAM x
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SUMMARY
A new technique by which thyrotrophic hormone (TSH) can be rapidly measured in vitro in the serum of euthyroid subjects and in human pituitary extracts is presented.
In euthyroid male subjects levels of TSH in serum ranged from 0 to 30·1 i.u. × 10−3/100ml., the mean value being 10·8. In normal menstruating women the mean value was 4·8 and the range 0 to 21·5 i.u. × 10−3/100 ml.
In human pituitary extracts, TSH could be detected in all fractions tested, but the amount of activity present was greatest in the gonadotrophin fraction. When the latter was separated into the FSH and LH components the TSH activity was found to be mainly associated with the LH fraction.
The specificity of the method has been examined by incubating thyroid tissue with other hormones of pituitary origin and measuring their effect on the response to TSH. It is concluded that any interference with the assay method produced by FSH and LH is due to their contamination with TSH.
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SUMMARY
Ion-exchange chromatography was used to further purify a human pituitary fraction rich in thyrotrophic and luteinizing hormone activities. Approximately twofold concentration of both activities was obtained by chromatography on IRC-50 at pH 7·5, but the hormones were not separated. Subsequent chromatography on DEAE-cellulose at pH 9·5 led to a tenfold concentration of the luteinizing hormone in a fraction practically free of thyrotrophic activity and to a fourfold concentration of the thyrotrophic hormone in a fraction still exhibiting substantial luteinizing hormone activity.
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A fractionation procedure used for the purification of glycoprotein hormones from pituitary glands of the human being (Stockell Hartree, 1966), horse (Stockell Hartree, Mills, Welch & Thomas, 1968) and chicken (Stockell Hartree & Cunningham, 1969) was also applied to acetone-dried dog pituitaries. The pituitaries were removed from dogs generally within 60 h of death. The glands were washed in acetone and stored in 20 volumes of acetone at 4 °C for several weeks. An acetone powder was prepared by drying the pituitaries in air and grinding them in a glass mortar. The yield of powder from 980 pituitaries was 10·3 g. This material was extracted with 6% ammonium acetate at pH 5·1 in 40% ethanol and the soluble extract chromatographed on a column of CM-cellulose equilibrated with 4 mm-ammonium acetate buffer at pH 5·5 as described previously (Stockell Hartree, 1966; Stockell Hartree et al. 1968; Stockell Hartree &
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SUMMARY
A prolonged study relating plasma testosterone levels to coitus is reported in one male subject. Testosterone levels in samples taken during and immediately after sexual intercourse were significantly higher than those found under resting conditions. A significant difference was not apparent between testosterone measurements taken before and after orgasm, and coitus did not appear to affect plasma luteinizing hormone levels. A marked nyctohemeral rhythm was observed in testosterone levels which tended to be higher early in the day. Masturbation had no significant effect on plasma testosterone levels in seven subjects.
