Search Results
You are looking at 1 - 2 of 2 items for
- Author: Kyuyong Han x
- Refine by access: All content x
Search for other papers by Jinwon Eo in
Google Scholar
PubMed
Search for other papers by Kyuyong Han in
Google Scholar
PubMed
Department of Biomedical Science and Technology, Department of Pathology and Immunology, Howard Hughes Medical Institute, Laboratory of Reproductive Biology and Infertility, RCTC, IBST, Konkuk University, 1 Hwayang-Dong, Kwangjin-Gu, Seoul 143-701, South Korea
Search for other papers by Kenneth M Murphy in
Google Scholar
PubMed
Search for other papers by Haengseok Song in
Google Scholar
PubMed
Search for other papers by Hyunjung Jade Lim in
Google Scholar
PubMed
Etv4, Etv1, and Etv5 are members of Etv4 subfamily of E26 transformation-specific (Ets) transcription factors that are known to influence a host of biological processes. We previously showed that Etv5, expressed in Sertoli cells, plays a crucial role in maintaining spermatogonial stem cell niche in the mouse testis. However, it is not yet known whether Etv4 family members are expressed in the ovary or play any role in ovarian functions. Here, we show that Etv5 and Etv4 are expressed in mouse ovaries in granulosa and cumulus cells during folliculogenesis. Both Etv5 and Etv4 mRNAs are also detected in cumulus–oocyte complexes (COCs) and denuded oocytes. Notably, Etv4 is highly expressed in the cumulus cells of ovulated COCs at 16-h post-human chorionic gonadotropin. Cyclooxygenase-2 (PTGS2), a rate-limiting enzyme for prostaglandin synthesis, is critical for oocyte maturation and ovulation. Since several putative Ets-binding sites are present in the PTGS2 promoter, we examined whether Etv5 influences Ptgs2 transcriptional activity. Indeed, we found that addition of Etv5 increases the transcriptional activity of the 3.2-kb mouse Ptgs2 promoter by 2.5-fold in luciferase reporter assays. Collectively, the results show that Etv4 and Etv5 are expressed in granulosa and cumulus cells during folliculogenesis and ovulation, suggesting that they influence cellular events in the ovary by regulating downstream genes such as Ptgs2.
Department of Biomedical Science and Technology, Institute of Biomedical Science and Technology, Konkuk University, 1 Hwayang-dong, Kwangjin-gu, Seoul 143-701, Korea
Laboratory of Reproductive Biology and Infertility, Cheil General Hospital, Women’s Healthcare Center, Seoul 100-380, Korea
Department of Biochemistry and Molecular Biology, UMDNJ-New Jersey Medical School, Newark, New Jersey 07101-1709, USA
Search for other papers by Kyuyong Han in
Google Scholar
PubMed
Department of Biomedical Science and Technology, Institute of Biomedical Science and Technology, Konkuk University, 1 Hwayang-dong, Kwangjin-gu, Seoul 143-701, Korea
Laboratory of Reproductive Biology and Infertility, Cheil General Hospital, Women’s Healthcare Center, Seoul 100-380, Korea
Department of Biochemistry and Molecular Biology, UMDNJ-New Jersey Medical School, Newark, New Jersey 07101-1709, USA
Search for other papers by Haengseok Song in
Google Scholar
PubMed
Department of Biomedical Science and Technology, Institute of Biomedical Science and Technology, Konkuk University, 1 Hwayang-dong, Kwangjin-gu, Seoul 143-701, Korea
Laboratory of Reproductive Biology and Infertility, Cheil General Hospital, Women’s Healthcare Center, Seoul 100-380, Korea
Department of Biochemistry and Molecular Biology, UMDNJ-New Jersey Medical School, Newark, New Jersey 07101-1709, USA
Search for other papers by Irene Moon in
Google Scholar
PubMed
Department of Biomedical Science and Technology, Institute of Biomedical Science and Technology, Konkuk University, 1 Hwayang-dong, Kwangjin-gu, Seoul 143-701, Korea
Laboratory of Reproductive Biology and Infertility, Cheil General Hospital, Women’s Healthcare Center, Seoul 100-380, Korea
Department of Biochemistry and Molecular Biology, UMDNJ-New Jersey Medical School, Newark, New Jersey 07101-1709, USA
Search for other papers by Robert Augustin in
Google Scholar
PubMed
Department of Biomedical Science and Technology, Institute of Biomedical Science and Technology, Konkuk University, 1 Hwayang-dong, Kwangjin-gu, Seoul 143-701, Korea
Laboratory of Reproductive Biology and Infertility, Cheil General Hospital, Women’s Healthcare Center, Seoul 100-380, Korea
Department of Biochemistry and Molecular Biology, UMDNJ-New Jersey Medical School, Newark, New Jersey 07101-1709, USA
Search for other papers by Kelle Moley in
Google Scholar
PubMed
Department of Biomedical Science and Technology, Institute of Biomedical Science and Technology, Konkuk University, 1 Hwayang-dong, Kwangjin-gu, Seoul 143-701, Korea
Laboratory of Reproductive Biology and Infertility, Cheil General Hospital, Women’s Healthcare Center, Seoul 100-380, Korea
Department of Biochemistry and Molecular Biology, UMDNJ-New Jersey Medical School, Newark, New Jersey 07101-1709, USA
Search for other papers by Melissa Rogers in
Google Scholar
PubMed
Department of Biomedical Science and Technology, Institute of Biomedical Science and Technology, Konkuk University, 1 Hwayang-dong, Kwangjin-gu, Seoul 143-701, Korea
Laboratory of Reproductive Biology and Infertility, Cheil General Hospital, Women’s Healthcare Center, Seoul 100-380, Korea
Department of Biochemistry and Molecular Biology, UMDNJ-New Jersey Medical School, Newark, New Jersey 07101-1709, USA
Search for other papers by Hyunjung Lim in
Google Scholar
PubMed
Various nuclear receptors form dimers to activate target genes via specific response elements located within promoters or enhancers. Retinoid X receptor (RXR) serves as a dimerization partner for many nuclear receptors including retinoic acid receptor (RAR) and peroxisome proliferator-activated receptor (PPAR). Dimers show differential preference towards directly repeated response elements with 1–5 nucleotide spacing, and direct repeat 1 (DR1) is a promiscuous element which recruits RAR/RXR, RXR/RXR, and PPAR/RXR in vitro. In the present investigation, we report identification of a novel RAR/RXR target gene which is regulated by DR1s in the promoter region. This gene, namely spermatocyte-specific marker (Ssm), recruits all the three combinations of nuclear receptors in vitro, but in vivo regulation is observed by trans-retinoic acid-activated RAR/RXR dimer. Indeed, chromatin immunoprecipitation experiment demonstrates binding of RARβ and RXRα in the promoter region of the Ssm. Interestingly, expression of Ssm is almost exclusively observed in spermatocytes in the adult mouse testis, where RA signaling is known to regulate developmental program of male germ cells. The results show that Ssm is a RAR/RXR target gene uniquely using DR1 and exhibits stage-specific expression in the mouse testis with potential function in later stages of spermatogenesis. This finding exemplifies usage of DR1s as retinoic acid response element (RARE) under a specific in vivo context.
