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SUMMARY
[4-14C]Oestradiol-17β, [4-14C]oestrone, Na[6,7-3H]oestradiol-17β-glucuronide and Na[6,7-3H]oestrone sulphate were injected into a Graafian follicle of two mares and a donkey after cannulation of the ovarian vein. Radioactivity was measured in follicular fluid and ovarian venous blood at different intervals after injection and in tissue and interstitial fluid at the end of the operation. Both free and conjugated oestrogens were retained to a great extent in the follicle, in the presence of constant production of endogenous oestrogens. Only a small portion of the oestrogen released from the follicle passed directly into the ovarian venous blood; a larger portion entered the effluent blood indirectly, through interstitial fluid and tissue. The conjugates, particularly the glucuronide, seemed to be excluded from the cells and accumulated in the interstitial fluid.
The role of binding of oestrogens to fluid proteins in the retention of oestrogen in follicular fluid is discussed.