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Fructose metabolism in the mammalian accessory sex organs is dependent on androgen (Mann, 1964). Fructose can be formed via either a phosphorylated or a nonphosphorylated metabolic pathway and several of the enzymes found in either of these pathways have been demonstrated to be dependent upon male sex hormone. Aldose reductase and ketose reductase are reduced after castration (Samuels, Harding & Mann, 1962); phosphatase (Stafford, Rubenstein & Meyer, 1949) and phosphofructokinase (Singhal, 1967) are similarly affected by androgens.
While fructose itself is readily lost from accessory sex structures after castration, less is known of the effects of androgen loss on the fructose phosphate esters such as fructose-6-phosphate (F-6-PO4) or fructose-1,6-diphosphate (F-1,6-diPO4). Fructose and fructose phosphate esters were therefore studied at short intervals after castration. Changes in the phosphatases were also investigated.
Tissues (anterior prostate or coagulating glands) were rapidly removed, frozen between sections of solid CO2, weighed and
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Coffey, Ichinose, Shimazaki & Williams (1968) and Ritter (1966) have demonstrated that 2 weeks after castration the concentration of ATP in the rat ventral prostate remains unchanged from normal levels but that 1 h after testosterone treatment dramatic reductions in ATP concentrations are observed. The cellular site of testosterone action upon ATP metabolism is unknown. In a complex tissue such as the prostate it is difficult, if not impossible, to examine separately the biochemistry of the epithelium and muscle; this can be effectively surmounted using the guinea-pig seminal vesicle. From this, a relatively pure preparation of epithelium can be obtained, and the biochemical sensitivity of these two cell lines may be compared. It is the purpose of this work to examine the effects of castration and testosterone replacement upon the levels of ATP in the epithelium and muscle of the guinea-pig seminal vesicle as well as in the prostate.
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