Our purposes in this study were to ascertain the effects of oestrogen on the accessory reproductive organs of male mice and rats, and to determine the capacity of androgen to counteract these effects.
The oestrogen, Amniotin, was furnished by Dr. J. A. Morrell of E. R. Squibb and Sons; and the androgen, testosterone, was supplied by Dr. Erwin Schwenk of the Schering Corporation.
Seventy-two rats and 71 mice have been used in this study. Thirty-one rats were given injections of oestrogen. The other 41 rats, litter-mates to the treated animals, received no injections. In the case of mice, 36 received only oestrogen, 13 were given oestrogen and testosterone, and 22 controls received no injections. The control mice in most cases were not litter-mates of injected animals.
Both testes were surgically removed from some of the animals. The immature rats were castrated on the 17th–24th day of life. The adult