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SUMMARY
Reduced triphosphopyridine nucleotide (TPNH) incubated with parathyroid gland extract (Injection Parathyroid, Lilly & Co.) for 6hr at pH 7·4 and at room temperature exhibits a reduction in u.v. absorption at 340 mμ. No correlation could be shown to exist between this decrease in absorbing capacity of TPNH at 340 mμ and the calcium-mobilizing activity of the extract.
Dialysis of the active extract against distilled water or treatment with ethanol-diethyl ether or with formaldehyde resulted in a marked reduction or virtual elimination of biological activity, and in a marked reduction in the ability of the treated preparation to affect the absorbing capacity of TPNH at 340 mμ. Inactivation of the calcium-mobilizing principle of the extract by pepsin resulted in a preparation which retained its ability to affect the u.v. absorption of TPNH. Dialysis of the pepsin-inactivated extract against distilled water or treatment with ethanol-diethyl ether decreased this spectrophotometric effect.