Search Results
You are looking at 1 - 1 of 1 items for
- Author: N. Skinner x
- Refine by access: All content x
Search for other papers by J. L. Vallet in
Google Scholar
PubMed
Search for other papers by P. J. Barker in
Google Scholar
PubMed
Search for other papers by G. E. Lamming in
Google Scholar
PubMed
Search for other papers by N. Skinner in
Google Scholar
PubMed
Search for other papers by N. S. Huskisson in
Google Scholar
PubMed
ABSTRACT
Ovine trophoblast protein-1 (oTP-1), stimulates the secretion of several proteins in explant culture of day-12 cyclic ovine endometrium. We partially purified and identified one of these proteins, an 11,000 Mr, pI approx. 6 protein by N-terminal amino acid sequencing and immunoprecipitation using antibody to human β2-microglobulin. The protein was purified from cultures of endometrium collected from day-16 pregnant ewes. The N-terminal amino acid sequence was 40–55% homologous to β2-microglobulin from a variety of species. Antibody to human β2-microglobulin immunoprecipitated the protein and another protein of similar molecular weight but more acidic pi. Using immunoprecipitation of radiolabelled proteins from culture, we demonstrated that oTP-1 increased production of this protein by 40% (P<0.05). We conclude that oTP-1 increases the secretion of a β2-microglobulin-like protein from day-12 non-pregnant endometrium in culture.