Search Results

You are looking at 1 - 1 of 1 items for

  • Author: P Forastieri x
  • Refine by access: All content x
Clear All Modify Search
V Pezzi
Search for other papers by V Pezzi in
Google Scholar
PubMed
Close
,
ML Panno
Search for other papers by ML Panno in
Google Scholar
PubMed
Close
,
R Sirianni
Search for other papers by R Sirianni in
Google Scholar
PubMed
Close
,
P Forastieri
Search for other papers by P Forastieri in
Google Scholar
PubMed
Close
,
I Casaburi
Search for other papers by I Casaburi in
Google Scholar
PubMed
Close
,
M Lanzino
Search for other papers by M Lanzino in
Google Scholar
PubMed
Close
,
V Rago
Search for other papers by V Rago in
Google Scholar
PubMed
Close
,
F Giordano
Search for other papers by F Giordano in
Google Scholar
PubMed
Close
,
C Giordano
Search for other papers by C Giordano in
Google Scholar
PubMed
Close
,
A Carpino
Search for other papers by A Carpino in
Google Scholar
PubMed
Close
, and
S Ando
Search for other papers by S Ando in
Google Scholar
PubMed
Close

Transient postnatal hypothyroidism in male rats induces a prolonged proliferation of immature Sertoli cells. This change in Sertoli cell replication at young ages is coincident with enhanced and prolonged aromatase activity that leads to a marked increase in the conversion of androgens into estrogens. Both events are drastically inhibited by tri-iodothyronine (T(3)) replacement either in vivo or in vitro. This study, after the immunolocalization of aromatase in cultured rat Sertoli cells, examined the effects elicited by T(3) on this enzyme, by simultaneously investigating three functional levels of aromatase: mRNA expression, protein content, and enzymatic activity. The immunolocalization of cytochrome P450 aromatase (P450 arom) was shown in the cytoplasm of cultured Sertoli cells from 15- and 21-day-old rats. Western blot analysis revealed an enhancement of aromatase protein content upon stimulation with N(6),2'-O-dibutyryladenosine-3':5'-cyclic monophosphate ((Bu)(2)cAMP) that was clearly down-regulated by T(3). The presence of a functional P450 arom protein in purified Sertoli cells was confirmed by the measurement of [(3)H]H(2)O released after incubation with [1 beta-(3)H]androst-4-ene-3,17-dione. With 100 nM T3, a decrease in both P450 arom mRNA levels and aromatase activity was observed. The aromatase enzymatic activity was strongly stimulated by (Bu)(2)cAMP and markedly down-regulated by T(3). In contrast, the strong increase in aromatase mRNA upon (Bu)(2)cAMP stimulation was apparently unaffected by T(3) administration. This paper shows how the identification of an altered transcript induced by T(3) coding for putative truncated and inactive aromatase protein might explain such a decrease in aromatase activity in T(3)-treated cells. On the basis of these results, it is concluded that at least two mechanisms could be involved in the down-regulatory effect of T(3) on aromatase activity in prepuberal Sertoli cells. The first mechanism is linked to a possible direct modulatory role for T(3) in the regulation of the aromatase promoter, whilst the second one is represented by the induction of altered transcripts coding for truncated and inactive aromatase proteins.

Free access