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SUMMARY
In order to determine the probable availability of protein-bound cortisol to the tissues, the establishment of equilibrium between transcortin-bound [3H]cortisol and unbound [14C]cortisol in plasma has been followed using Sephadex gel filtration. Association of cortisol with transcortin was rapid; dissociation of the steroid-protein complex was comparatively slow; when the binding-sites were nearly saturated the half-dissociation times of [3H]cortisol were 25 min. at 4°, 1 min. at 22° and about 10 sec. at 37°.
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SUMMARY
The binding of plasma cortisol to transcortin at 37° c was studied in normal men and in non-pregnant and pregnant women. The mean concentrations of transcortin were 8, 11·5 and 14 × 10−7 moles/l. and the percentages of the binding sites occupied by cortisol were 45, 24 and 40%, respectively. The mean values of the equilibrium constants were 2·9, 1·8 and 4·6 × 107 l./mole, suggesting that there might be a qualitative difference in transcortin from the three groups. The mean concentration of diffusible cortisol was 1·12 μg./100 ml. in men and 0·65 μg./100 ml. in non-pregnant and pregnant women.
The significance of these findings is discussed.
