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The purpose of this communication is to report the presence of cornified cells in the vaginal smear of the rat before pro-oestrus.
The cytological changes in the vaginal smear of the rat between the epithelial stage of pro-oestrus and the complete leucocyte infiltration of dioestrus have been well described (e.g. Long & Evans, 1922; Astwood, 1939; Young, Boling & Blandau, 1941; Hartman, 1944; Mandl, 1951). However, virtually no information is available about the interval between dioestrus and pro-oestrus except for the fact that the leucocytes are replaced by the numerous, small, round, nucleated epithelial cells that typify pro-oestrus. A stage of 'cell paucity' in late dioestrus was noted by Astwood (1939) and later confirmed by Hartman (1944). Mandl (1951) termed the last 7 hr. before pro-oestrus 'late dioestrum' and stated that cornified cells are not present in smears taken during this stage of the cycle. There is no additional information