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W. I. MORSE, A. F. CLARK and S. C. MACLEOD

SUMMARY

Tracer doses (1 μg.) of tritiated oestradiol-17β were administered to a healthy 36-year-old woman on the 19th day of a 29-day ovulatory cycle and the 6th day of the following cycle. Urine was collected for 5 days after each injection and was assayed for oestradiol, oestrone, oestriol and for radioactivity in these oestrogen fractions. The data were used to calculate the size of the oestradiol-plus-oestrone miscible body pool (approx. 227 μg. on day 19 and 61 μg. on day 6).

There was evidence that the label was mixed throughout most of the pool within a few hours of its injection.

The percentages of injected radioactivity excreted with the three oestrogens studied were similar for both studies. The oestriol recovery was greater than that reported by other investigators following injections of massive doses of oestradiol. The calculated endogenous production rate of oestradiol was approximately 165 μg./day on day 19 and 93 μg./day on day 6. The findings also indicated that oestrone and oestradiol were the source of essentially all endogenously produced oestriol.

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J. B. BROWN, S. C. MacLEOD, CAROLINE MACNAUGHTAN, MARGERY A. SMITH and BARBARA SMYTH

SUMMARY

A semi-automatic extractor was developed which processes 12 urines at a time to a stage where 'total' oestrogens can be measured by colorimetry using the Kober reaction (late pregnancy urines) or by fluorimetry using the Kober—Ittrich procedure (non-pregnancy and early pregnancy urines). One worker can complete 12 analyses in 3½ hr. or 24–36 in a working day. The results obtained at oestrogen levels above 1 mg./24-hr. urine were the same as those obtained by a method specific for oestriol. The results obtained from non-pregnancy urines were compared with the sums of oestriol, oestrone and oestradiol obtained by the method of Brown (1955). The mean ratio (± s.d.) of the two values was 1·22 ± 0·31. The comparisons indicated that the short procedure was the more reliable method at levels below 5 μg./24-hr. urine. Values for normal subjects are given. The methods appear to be entirely suitable for assessing oestrogen production by the ovaries, testes, adrenals and placenta.