Search Results

You are looking at 1 - 2 of 2 items for

  • Author: STRETTON YOUNG x
Clear All Modify Search
Restricted access

STRETTON YOUNG and ANN E. NELSTROP

The secretion of milk proteins is one of the functions of the mammary gland during pregnancy and lactation and it may also occur to some extent during certain pathological processes. If it were possible to detect these proteins consistently and locate them accurately our knowledge of mammary gland physiology and pathology would be much improved. We have used an immunofluorescent method for this purpose and this preliminary communication records some of our results.

Milk was collected from lactating rats when the young were 10–15 days old, it was frozen immediately and stored at − 20°. When enough milk had been collected it was allowed to thaw, the fat was removed and casein was separated by high-speed centrifugation or by acid precipitation at about pH 4. Rabbits were immunized against this rat casein by a single s.c. injection of antigen plus Freund's adjuvant followed by booster i.m. injections of antigen alone,

Restricted access

D. Y. WANG, STRETTON YOUNG and R. D. BULBROOK

SUMMARY

(1) The incorporation of [1,2-3H]testosterone in vivo into various tissues of virgin, pregnant, post-partum and tumour-bearing female rats was studied.

(2) In virgin female rats the clearance of radioactivity from mesenteric fat, mammary gland, uterus, spleen, lung and blood was similar. This similarity in the rates of clearance of radioactivity for all the tissues examined was also found for the tissues of pregnant, post-partum, and tumour-bearing rats.

(3) After the administration of [1,2-3H]testosterone different amounts of radioactivity were found in each of the tissues examined. In virgin rats the levels of incorporation were fat > uterus ≥ mammary gland > lung > blood ≥ spleen. This pattern was also obtained in post-partum and tumour-bearing animals; the tumours in the latter behaved in a similar way to normal mammary glands. In the pregnant rat, the foetus incorporated the least amount of radioactivity.