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In order to obtain information on the biochemical viability of oestrogen-dependent tissues in culture, studies have been carried out on the interconversion of oestrone and oestradiol by rabbit uterus in culture and the fate of the hormones in the cultured tissue (Russell & Thomas, 1973a, b). These studies are now being extended to human tissue. Significant conversion of oestrone to oestradiol has been found in human breast tumours maintained in organ culture (Wilcox & Thomas, 1972). A similar approach has been used in the present study to investigate oestrogen metabolism in tissues of the female reproductive tract during culture.
[6, 7-3H]Oestrone (30 Ci/mmol) and [6, 7-3H]oestradiol-17β (36 Ci/mmol) (Radiochemical Centre, Amersham) were checked routinely by chromatography. Stock solutions were prepared in ethanol such that 5 μl dispensed into the culture medium (5 ml) gave a final concentration of 10−9 mol/l. In experiments using 10
