Search Results
You are looking at 1 - 4 of 4 items for
- Author: T. HIROSE x
- Refine by access: All content x
Search for other papers by T. HIROSE in
Google Scholar
PubMed
Search for other papers by I. MATSUMOTO in
Google Scholar
PubMed
Search for other papers by T. AIKAWA in
Google Scholar
PubMed
SUMMARY
The steroidogenic effect of histamine in isolated adrenocortical cells of the dog was investigated in the presence of prostaglandin E2 (PGE2) and/or dibutyryl cyclic AMP (dbcAMP) in the medium. The effect of histamine, in combination with PGE2, was less than their total individual effects in the production of cortisol, but not of corticosterone. With dbcAMP the effect was just equal to them. However, the combination of histamine, PGE2 and dbcAMP showed an increase twice that of their total individual effects in the production of both steroids. These results indicate that, in the dog, histamine, PGE2 and dbcAMP may act synergistically in the adrenocortical cells.
Search for other papers by T. HIROSE in
Google Scholar
PubMed
Search for other papers by I. MATSUMOTO in
Google Scholar
PubMed
Search for other papers by T. AIKAWA in
Google Scholar
PubMed
Department of Physiology, Nagasaki University School of Medicine, Nagasaki, Japan
(Received 2 September 1977)
In the dog, intravenous injection of histamine produces a marked increase in adrenocortical secretion (Suzuki, Hirai, Yoshio, Kurouji & Yamashita, 1963; Papp, Stark, Ács & Varga, 1964; Asano, 1966; Katsuki, Ito, Watanabe, lino, Yuji & Kondo, 1967; Tanigawa, 1967; Narita, 1971; Yamashita, Shimizu, Mieno & Kawao, 1973; Hirose, Matsumoto & Suzuki, 1976; Hirose, Matsumoto, Aikawa & Suzuki, 1977). The adrenocortical response of the dog to histamine was found to be markedly reduced, although not completely eliminated, by hypophysectomy (Hirose et al. 1976, 1977). This indicates that the response, although dependent for the main part on the pituitary gland, may involve a direct effect of histamine on the adrenal cortex or some other extrapituitary factor. In the present study, a direct stimulatory effect of histamine on the adrenal cortex was examined by evaluating the production of cortisol
Search for other papers by T. HIROSE in
Google Scholar
PubMed
Search for other papers by I. MATSUMOTO in
Google Scholar
PubMed
Search for other papers by T. AIKAWA in
Google Scholar
PubMed
Search for other papers by T. SUZUKI in
Google Scholar
PubMed
Department of Physiology, Nagasaki University School of Medicine, Nagasaki, Japan
(Received 5 January 1977)
A systemic administration of histamine to intact dogs markedly increases the adrenocortical secretion (Suzuki, Hirai, Yoshio, Kurouji & Yamashita, 1963; Papp, Stark, Acs & Varga, 1964; Asano, 1966; Katsuki, Ito, Watanabe, Iino, Yuji & Kondo, 1967; Tanigawa, 1967; Yamashita, Shimizu, Mieno & Kawao, 1973). This effect was found to be completely abolished by hypophysectomy (Asano, 1966). In the experiments by Tanigawa (1967), however, a slight increase in adrenocortical secretion in response to histamine was observed in some hypophysectomized dogs. In the present work a direct adrenocortical stimulatory effect of histamine was examined by evaluating the adrenal secretion of cortisol and corticosterone in response to histamine in hypophysectomized dogs.
Thirteen mongrel male dogs weighing 10·0–16·8 kg were used; four of them were intact, and the others were hypophysectomized. Under sodium pentobarbitone (25 mg/kg, i.v.) anaesthesia, hypophysectomy was
Search for other papers by T. SUZUKI in
Google Scholar
PubMed
Search for other papers by K. HIRAI in
Google Scholar
PubMed
Search for other papers by H. YOSHIO in
Google Scholar
PubMed
Search for other papers by K-I. KUROUJI in
Google Scholar
PubMed
Search for other papers by T. HIROSE in
Google Scholar
PubMed
That eserine and atropine stimulate the pituitary-adrenocortical system has been proved only by indirect evaluation such as the rat adrenal ascorbic acid depletion test (Dordoni & Fortier, 1950). In the experiments of Harwood & Mason (1956) eserine was injected intravenously in a dose of 0·05 mg./kg. body weight into unanaesthetized dogs. Although a marked eosinopenia was observed, no definite rise in the level of 17-hydroxycorticosteroids (17-OHCS) in peripheral blood was found after administration of eserine. In the present study attempts were made to evaluate directly the effect of eserine and atropine on adrenal 17-OHCS secretion in unanaesthetized dogs.
Six mongrel dogs weighing between 11 and 15 kg. were used. Samples of adrenal venous blood were taken from the unanaesthetized animals by a modification of the method of Satake, Sugawara & Watanabe (1927) (see Suzuki, Hirai, Yoshio, Kurouji & Yamashita, 1963).
The observations were started about 18 hr. after completion of