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Makoto Kawasaki
Makoto Kawasaki
Departments of Physiology and
Orthopaedics, School of Medicine, University of Occupational and Environmental Health, Kitakyushu 807-8555, Japan
Department of Physiology, Jichi Medical School, Tochigi 329-0498, Japan
Department of Third Internal Medicine, Miyazaki University, Miyazaki 889-1692, Japan
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Orthopaedics, School of Medicine, University of Occupational and Environmental Health, Kitakyushu 807-8555, Japan
Department of Physiology, Jichi Medical School, Tochigi 329-0498, Japan
Department of Third Internal Medicine, Miyazaki University, Miyazaki 889-1692, Japan
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Tatsushi Onaka
Tatsushi Onaka
Departments of Physiology and
Orthopaedics, School of Medicine, University of Occupational and Environmental Health, Kitakyushu 807-8555, Japan
Department of Physiology, Jichi Medical School, Tochigi 329-0498, Japan
Department of Third Internal Medicine, Miyazaki University, Miyazaki 889-1692, Japan
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Orthopaedics, School of Medicine, University of Occupational and Environmental Health, Kitakyushu 807-8555, Japan
Department of Physiology, Jichi Medical School, Tochigi 329-0498, Japan
Department of Third Internal Medicine, Miyazaki University, Miyazaki 889-1692, Japan
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Masamitsu Nakazato
Masamitsu Nakazato
Departments of Physiology and
Orthopaedics, School of Medicine, University of Occupational and Environmental Health, Kitakyushu 807-8555, Japan
Department of Physiology, Jichi Medical School, Tochigi 329-0498, Japan
Department of Third Internal Medicine, Miyazaki University, Miyazaki 889-1692, Japan
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Orthopaedics, School of Medicine, University of Occupational and Environmental Health, Kitakyushu 807-8555, Japan
Department of Physiology, Jichi Medical School, Tochigi 329-0498, Japan
Department of Third Internal Medicine, Miyazaki University, Miyazaki 889-1692, Japan
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Jun Saito
Jun Saito
Departments of Physiology and
Orthopaedics, School of Medicine, University of Occupational and Environmental Health, Kitakyushu 807-8555, Japan
Department of Physiology, Jichi Medical School, Tochigi 329-0498, Japan
Department of Third Internal Medicine, Miyazaki University, Miyazaki 889-1692, Japan
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Orthopaedics, School of Medicine, University of Occupational and Environmental Health, Kitakyushu 807-8555, Japan
Department of Physiology, Jichi Medical School, Tochigi 329-0498, Japan
Department of Third Internal Medicine, Miyazaki University, Miyazaki 889-1692, Japan
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Takashi Mera
Takashi Mera
Departments of Physiology and
Orthopaedics, School of Medicine, University of Occupational and Environmental Health, Kitakyushu 807-8555, Japan
Department of Physiology, Jichi Medical School, Tochigi 329-0498, Japan
Department of Third Internal Medicine, Miyazaki University, Miyazaki 889-1692, Japan
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Orthopaedics, School of Medicine, University of Occupational and Environmental Health, Kitakyushu 807-8555, Japan
Department of Physiology, Jichi Medical School, Tochigi 329-0498, Japan
Department of Third Internal Medicine, Miyazaki University, Miyazaki 889-1692, Japan
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Hirofumi Hashimoto
Hirofumi Hashimoto
Departments of Physiology and
Orthopaedics, School of Medicine, University of Occupational and Environmental Health, Kitakyushu 807-8555, Japan
Department of Physiology, Jichi Medical School, Tochigi 329-0498, Japan
Department of Third Internal Medicine, Miyazaki University, Miyazaki 889-1692, Japan
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Orthopaedics, School of Medicine, University of Occupational and Environmental Health, Kitakyushu 807-8555, Japan
Department of Physiology, Jichi Medical School, Tochigi 329-0498, Japan
Department of Third Internal Medicine, Miyazaki University, Miyazaki 889-1692, Japan
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Hiroaki Fujihara
Hiroaki Fujihara
Departments of Physiology and
Orthopaedics, School of Medicine, University of Occupational and Environmental Health, Kitakyushu 807-8555, Japan
Department of Physiology, Jichi Medical School, Tochigi 329-0498, Japan
Department of Third Internal Medicine, Miyazaki University, Miyazaki 889-1692, Japan
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Orthopaedics, School of Medicine, University of Occupational and Environmental Health, Kitakyushu 807-8555, Japan
Department of Physiology, Jichi Medical School, Tochigi 329-0498, Japan
Department of Third Internal Medicine, Miyazaki University, Miyazaki 889-1692, Japan
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Nobukazu Okimoto
Nobukazu Okimoto
Departments of Physiology and
Orthopaedics, School of Medicine, University of Occupational and Environmental Health, Kitakyushu 807-8555, Japan
Department of Physiology, Jichi Medical School, Tochigi 329-0498, Japan
Department of Third Internal Medicine, Miyazaki University, Miyazaki 889-1692, Japan
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Orthopaedics, School of Medicine, University of Occupational and Environmental Health, Kitakyushu 807-8555, Japan
Department of Physiology, Jichi Medical School, Tochigi 329-0498, Japan
Department of Third Internal Medicine, Miyazaki University, Miyazaki 889-1692, Japan
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Hideo Ohnishi
Hideo Ohnishi
Departments of Physiology and
Orthopaedics, School of Medicine, University of Occupational and Environmental Health, Kitakyushu 807-8555, Japan
Department of Physiology, Jichi Medical School, Tochigi 329-0498, Japan
Department of Third Internal Medicine, Miyazaki University, Miyazaki 889-1692, Japan
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Orthopaedics, School of Medicine, University of Occupational and Environmental Health, Kitakyushu 807-8555, Japan
Department of Physiology, Jichi Medical School, Tochigi 329-0498, Japan
Department of Third Internal Medicine, Miyazaki University, Miyazaki 889-1692, Japan
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Toshitaka Nakamura
Toshitaka Nakamura
Departments of Physiology and
Orthopaedics, School of Medicine, University of Occupational and Environmental Health, Kitakyushu 807-8555, Japan
Department of Physiology, Jichi Medical School, Tochigi 329-0498, Japan
Department of Third Internal Medicine, Miyazaki University, Miyazaki 889-1692, Japan
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Orthopaedics, School of Medicine, University of Occupational and Environmental Health, Kitakyushu 807-8555, Japan
Department of Physiology, Jichi Medical School, Tochigi 329-0498, Japan
Department of Third Internal Medicine, Miyazaki University, Miyazaki 889-1692, Japan
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Yoichi Ueta
Yoichi Ueta
Departments of Physiology and
Orthopaedics, School of Medicine, University of Occupational and Environmental Health, Kitakyushu 807-8555, Japan
Department of Physiology, Jichi Medical School, Tochigi 329-0498, Japan
Department of Third Internal Medicine, Miyazaki University, Miyazaki 889-1692, Japan
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Orthopaedics, School of Medicine, University of Occupational and Environmental Health, Kitakyushu 807-8555, Japan
Department of Physiology, Jichi Medical School, Tochigi 329-0498, Japan
Department of Third Internal Medicine, Miyazaki University, Miyazaki 889-1692, Japan
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We examined the effects of i.c.v. administration of neuro-peptide W-30 (NPW30) on plasma arginine vasopressin (AVP) and plasma oxytocin (OXT) using RIA. The induction of c-fos mRNA, AVP heteronuclear (hn)RNA, and c-Fos protein (Fos) in the supraoptic nucleus (SON) and paraventricular nucleus (PVN) of rats were also investigated using in situ hybridization histochemistry for c-fos mRNA and AVP hnRNA, and immunohistochemistry for Fos. Both plasma AVP and OXT were significantly increased at 5 and 15 min after i.c.v. administration of NPW30 (2.8 nmol/rat). In situ hybridization histochemistry revealed that the induction of c-fos mRNA and AVP hnRNA in the SON and PVN were significantly increased 15, 30, and 60 min after i.c.v. administration of NPW30 (1.4 nmol/rat). Dual immunostaining for Fos/AVP and Fos/OXT revealed that both AVP-like immunoreactive (LI) cells and OXT-LI cells exhibited nuclear Fos-LI in the SON and PVN, 90 min after i.c.v. administration of NPW30 (2.8 nmol/rat). These results suggest that central NPW30 may be involved in the regulation of secretion of AVP and OXT in the magnocellular neurosecretory cells in the SON and PVN.
Akiko Katoh
Akiko Katoh
Departments of, Physiology, Otorhynolaryngology, Department of Physiology, Section on Neural Gene Expression, Department of Cellular Neurophysiology, Department of Anatomy and Neurobiology, Molecular Neuroendocrinology Research Group, School of Medicine, University of Occupational and Environmental Health, 1-1 Iseigaoka, Yahatanishi-ku, Kitakyushu 807-8555, Japan
Departments of, Physiology, Otorhynolaryngology, Department of Physiology, Section on Neural Gene Expression, Department of Cellular Neurophysiology, Department of Anatomy and Neurobiology, Molecular Neuroendocrinology Research Group, School of Medicine, University of Occupational and Environmental Health, 1-1 Iseigaoka, Yahatanishi-ku, Kitakyushu 807-8555, Japan
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Departments of, Physiology, Otorhynolaryngology, Department of Physiology, Section on Neural Gene Expression, Department of Cellular Neurophysiology, Department of Anatomy and Neurobiology, Molecular Neuroendocrinology Research Group, School of Medicine, University of Occupational and Environmental Health, 1-1 Iseigaoka, Yahatanishi-ku, Kitakyushu 807-8555, Japan
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Hiroaki Fujihara
Hiroaki Fujihara
Departments of, Physiology, Otorhynolaryngology, Department of Physiology, Section on Neural Gene Expression, Department of Cellular Neurophysiology, Department of Anatomy and Neurobiology, Molecular Neuroendocrinology Research Group, School of Medicine, University of Occupational and Environmental Health, 1-1 Iseigaoka, Yahatanishi-ku, Kitakyushu 807-8555, Japan
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Toyoaki Ohbuchi
Toyoaki Ohbuchi
Departments of, Physiology, Otorhynolaryngology, Department of Physiology, Section on Neural Gene Expression, Department of Cellular Neurophysiology, Department of Anatomy and Neurobiology, Molecular Neuroendocrinology Research Group, School of Medicine, University of Occupational and Environmental Health, 1-1 Iseigaoka, Yahatanishi-ku, Kitakyushu 807-8555, Japan
Departments of, Physiology, Otorhynolaryngology, Department of Physiology, Section on Neural Gene Expression, Department of Cellular Neurophysiology, Department of Anatomy and Neurobiology, Molecular Neuroendocrinology Research Group, School of Medicine, University of Occupational and Environmental Health, 1-1 Iseigaoka, Yahatanishi-ku, Kitakyushu 807-8555, Japan
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Departments of, Physiology, Otorhynolaryngology, Department of Physiology, Section on Neural Gene Expression, Department of Cellular Neurophysiology, Department of Anatomy and Neurobiology, Molecular Neuroendocrinology Research Group, School of Medicine, University of Occupational and Environmental Health, 1-1 Iseigaoka, Yahatanishi-ku, Kitakyushu 807-8555, Japan
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Tatsushi Onaka
Tatsushi Onaka
Departments of, Physiology, Otorhynolaryngology, Department of Physiology, Section on Neural Gene Expression, Department of Cellular Neurophysiology, Department of Anatomy and Neurobiology, Molecular Neuroendocrinology Research Group, School of Medicine, University of Occupational and Environmental Health, 1-1 Iseigaoka, Yahatanishi-ku, Kitakyushu 807-8555, Japan
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W Scott Young III
W Scott Young III
Departments of, Physiology, Otorhynolaryngology, Department of Physiology, Section on Neural Gene Expression, Department of Cellular Neurophysiology, Department of Anatomy and Neurobiology, Molecular Neuroendocrinology Research Group, School of Medicine, University of Occupational and Environmental Health, 1-1 Iseigaoka, Yahatanishi-ku, Kitakyushu 807-8555, Japan
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Govindan Dayanithi
Govindan Dayanithi
Departments of, Physiology, Otorhynolaryngology, Department of Physiology, Section on Neural Gene Expression, Department of Cellular Neurophysiology, Department of Anatomy and Neurobiology, Molecular Neuroendocrinology Research Group, School of Medicine, University of Occupational and Environmental Health, 1-1 Iseigaoka, Yahatanishi-ku, Kitakyushu 807-8555, Japan
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Yuka Yamasaki
Yuka Yamasaki
Departments of, Physiology, Otorhynolaryngology, Department of Physiology, Section on Neural Gene Expression, Department of Cellular Neurophysiology, Department of Anatomy and Neurobiology, Molecular Neuroendocrinology Research Group, School of Medicine, University of Occupational and Environmental Health, 1-1 Iseigaoka, Yahatanishi-ku, Kitakyushu 807-8555, Japan
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Mitsuhiro Kawata
Mitsuhiro Kawata
Departments of, Physiology, Otorhynolaryngology, Department of Physiology, Section on Neural Gene Expression, Department of Cellular Neurophysiology, Department of Anatomy and Neurobiology, Molecular Neuroendocrinology Research Group, School of Medicine, University of Occupational and Environmental Health, 1-1 Iseigaoka, Yahatanishi-ku, Kitakyushu 807-8555, Japan
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Hitoshi Suzuki
Hitoshi Suzuki
Departments of, Physiology, Otorhynolaryngology, Department of Physiology, Section on Neural Gene Expression, Department of Cellular Neurophysiology, Department of Anatomy and Neurobiology, Molecular Neuroendocrinology Research Group, School of Medicine, University of Occupational and Environmental Health, 1-1 Iseigaoka, Yahatanishi-ku, Kitakyushu 807-8555, Japan
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Hiroki Otsubo
Hiroki Otsubo
Departments of, Physiology, Otorhynolaryngology, Department of Physiology, Section on Neural Gene Expression, Department of Cellular Neurophysiology, Department of Anatomy and Neurobiology, Molecular Neuroendocrinology Research Group, School of Medicine, University of Occupational and Environmental Health, 1-1 Iseigaoka, Yahatanishi-ku, Kitakyushu 807-8555, Japan
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Hideaki Suzuki
Hideaki Suzuki
Departments of, Physiology, Otorhynolaryngology, Department of Physiology, Section on Neural Gene Expression, Department of Cellular Neurophysiology, Department of Anatomy and Neurobiology, Molecular Neuroendocrinology Research Group, School of Medicine, University of Occupational and Environmental Health, 1-1 Iseigaoka, Yahatanishi-ku, Kitakyushu 807-8555, Japan
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David Murphy
David Murphy
Departments of, Physiology, Otorhynolaryngology, Department of Physiology, Section on Neural Gene Expression, Department of Cellular Neurophysiology, Department of Anatomy and Neurobiology, Molecular Neuroendocrinology Research Group, School of Medicine, University of Occupational and Environmental Health, 1-1 Iseigaoka, Yahatanishi-ku, Kitakyushu 807-8555, Japan
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Yoichi Ueta
Yoichi Ueta
Departments of, Physiology, Otorhynolaryngology, Department of Physiology, Section on Neural Gene Expression, Department of Cellular Neurophysiology, Department of Anatomy and Neurobiology, Molecular Neuroendocrinology Research Group, School of Medicine, University of Occupational and Environmental Health, 1-1 Iseigaoka, Yahatanishi-ku, Kitakyushu 807-8555, Japan
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We have generated rats bearing an oxytocin (OXT)-enhanced cyan fluorescent protein (eCFP) fusion transgene designed from a murine construct previously shown to be faithfully expressed in transgenic mice. In situ hybridisation histochemistry revealed that the Oxt–eCfp fusion gene was expressed in the supraoptic nucleus (SON) and the paraventricular nucleus (PVN) in these rats. The fluorescence emanating from eCFP was observed only in the SON, the PVN, the internal layer of the median eminence and the posterior pituitary (PP). In in vitro preparations, freshly dissociated cells from the SON and axon terminals showed clear eCFP fluorescence. Immunohistochemistry for OXT and arginine vasopressin (AVP) revealed that the eCFP fluorescence co-localises with OXT immunofluorescence, but not with AVP immunofluorescence in the SON and the PVN. Although the expression levels of the Oxt–eCfp fusion gene in the SON and the PVN showed a wide range of variations in transgenic rats, eCFP fluorescence was markedly increased in the SON and the PVN, but decreased in the PP after chronic salt loading. The expression of the Oxt gene was significantly increased in the SON and the PVN after chronic salt loading in both non-transgenic and transgenic rats. Compared with wild-type animals, euhydrated and salt-loaded male and female transgenic rats showed no significant differences in plasma osmolality, sodium concentration and OXT and AVP levels, suggesting that the fusion gene expression did not disturb any physiological processes. These results suggest that our new transgenic rats are a valuable new tool to identify OXT-producing neurones and their terminals.
