parameters. Figure 1 MicroCT and histological analysis of Scarb1 -null mouse vertebrae. (A) Representative 3D renderings of 2- and 4-month-old mouse fourth lumbar vertebrae bone volumes of interest (VOI) and representative 3D renderings of VOI. VOI was
Corine Martineau, Louise Martin-Falstrault, Louise Brissette and Robert Moreau
Raylene A Reimer, Gary J Grover, Lee Koetzner, Roland J Gahler, Michael R Lyon and Simon Wood
, Hologic, Bedford, MA, USA). Tissue collection and histology Following the cardiac blood draw, one kidney, one lobe of the liver, and a section of the distal ileum were snap–frozen for later DPP4 analysis (DPP4 activity in kidney and Dpp4 mRNA levels
Amy R Quinn, Cynthia L Blanco, Carla Perego, Giovanna Finzi, Stefano La Rosa, Carlo Capella, Rodolfo Guardado-Mendoza, Francesca Casiraghi, Amalia Gastaldelli, Marney Johnson, Edward J Dick Jr and Franco Folli
, and adult pancreatic tissues were obtained postmortem for routine histological staining. The weight of each animal and of the pancreatic tissue was recorded at necropsy and pancreas as a percentage of body weight was calculated. The majority of the
Marina Komrakova, Carsten Krischek, Michael Wicke, Stephan Sehmisch, Mohammad Tezval, Maximillian Rohrberg, Thomas Brandsch, Klaus M Stuermer and Ewa K Stuermer
carefully excised, weighted, pinned at both ends, and cut in the middle across the muscle. Thereafter, muscle samples were removed from the center of the ML, MG, and MS, frozen directly in liquid nitrogen, and stored at −80 °C until either histological or
Yi Lin and Zhongjie Sun
kidney from each mouse was fixed with 4% paraformaldehyde in PBS for 24 h. The kidneys were set in a plane perpendicular to the long axis and were embedded in paraffin for histological and immunohistochemical analysis. Measurements of albumin Urinary
J. BORNSTEIN, C. H. GRAY and DELPHINE M. V. PARROTT
An acid acetone extract of human plasma has been shown to be active in partially maintaining the weight and histological structure of the adrenals of hypophysectomized rats. As extracts of this type have been previously shown to be active in the Sayer's test, it appears that ACTH may be demonstrable in human plasma.
S. Y. CHOW and D. M. WOODBURY
[14C]Inulin, 35SO4 2- and 36Cl- spaces, 131I- thyroid: serum (T:S) ratio, histological measurements of follicles and electrolyte content of the thyroid gland were determined in male rats 3–17 weeks of age. The thyroid [14C]inulin space of animals of different ages was not significantly different. The thyroid 35SO4 2- space, however, varied with age. The youngest rats had the largest 35SO4 2- space. Histological measurements indicated that in the thyroid follicles the epithelial cells were much thinner and the luminal volume was much larger in the younger rats than in older animals. In the older rats, the 35SO4 2- space was equal to the sum of [14C]inulin (stromal) and the histologically determined luminal spaces. In the younger animals, although the 35SO4 2- space was larger than in the older animals, it was still not large enough to equal the sum of the volumes determined by the [14C]inulin and the direct histological measurements of the lumen. This is due to the high colloid content in the follicular lumen of the young rats. The lower thyroid 131I- T:S ratio in younger rats also indicated that the thyroid gland was less active than in older animals. The volume of distribution of thyroid Cl- calculated from the chemically determined data of the plasma and of the thyroid tissue concentrations of rats of different ages was the same as that determined by 36Cl-.
A. WRIGHT and I. CHESTER JONES
The anatomy and histology of the adrenal glands of the West African lizard (Agama agama L.) and of the common grass snake (Natrix natrix L.) are described.
In lizards, 30 days after hypophysectomy, there was considerable degeneration, with a 60% loss in weight, of the adrenal. The chromaffin cells underwent no histological change. Some cortical cells contained large sudanophilic, Schultz-positive lipid droplets, some nuclei remaining normal, others showing intense basophilia and shrinkage. In other cells, the cytoplasm was reduced to a small acidophilic patch, with pyknotic nuclei. The sodium and potassium contents of the blood and muscle of these hypophysectomized animals were within the normal range.
In snakes, the successive histological stages in the degeneration of the adrenal cortex 9–11, 20 and 30–39 days after hypophysectomy are described. Injection of mammalian ACTH allowed the adrenal to maintain a normal histological appearance in hypophysectomized snakes. In addition, these animals, and normal animals similarly injected, showed degenerative areas in the cortex, attributable to over-stimulation. Injections of cortisone and DCA into unoperated animals were followed by degeneration of the adrenal cortex. After unilateral adrenalectomy, the contralateral adrenal was hypertrophic.
The sodium and potassium contents of blood and muscle, together with the water content of the latter, were obtained in all groups of animals. Despite the histological changes induced in the adrenal cortex by the various experimental procedures, there were no profound changes in the distribution of salt-electrolytes in any of the animals. The sodium content of muscle of normal snakes was about twice the value found in vertebrate muscle in general.
The results are discussed in the light of present-day knowledge of the adrenal cortex in vertebrates, especially of the gland in mammals.
G M Ledda-Columbano, A Perra, M Pibiri, F Molotzu and A Columbano
). Histology and immunohistochemistry Pancreas sections were fixed in 10% buffered formalin and processed for staining with hematoxylin–eosin or immunohistochemistry immediately after death. The remaining pancreas was snap-frozen in liquid nitrogen
Erica Sarchielli, Paolo Comeglio, Sandra Filippi, Ilaria Cellai, Giulia Guarnieri, Daniele Guasti, Elena Rapizzi, Giulia Rastrelli, Daniele Bani, Gabriella Vannelli, Linda Vignozzi, Annamaria Morelli and Mario Maggi
mixed composition of type I/type II fibers and therefore useful for studying whether particular conditions could induce changes toward another fiber type. Histological evaluation of HFD muscle samples showed a reduction of slow/type I fibers, which are