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K H Liu, J C Huang and J D Godkin

Abstract

Caprine chorion, allantois and amnion from days 23, 28, 35, 39 and 45, and yolk sac from day 23 of pregnancy were isolated by dissection and cultured for 24 h in modified minimum essential medium in the presence of [35S] methionine. De novo-synthesized proteins released into the culture medium were analyzed by two-dimensional PAGE and fluorography. Patterns of protein production by these isolated extraembryonic membranes remained relatively unchanged from days 23 to 45 of pregnancy. Electrophoretic profiles of proteins synthesized by allantois and amnion were identical but distinct from that produced by chorion. Yolk sac was the major source of serum-like proteins. An acidic (pI 5·3–6·3) 22 kDa protein, which consisted of four isoelectric variants, was produced by all extraembryonic membranes and demonstrated to immunoreact with antiserum produced against bovine placental retinol-binding protein (RBP). Limited N-terminal sequence analysis of one major isoform indicated that the protein had complete homology with bovine RBP over the first 15 amino acids. Immunoreactive RBP was localized in epithelial cells lining the chorion, allantois and amnion. In this study, we have characterized and compared protein production by isolated extraembryonic membranes through days 23 to 45 of pregnancy and identified the 22 kDa protein as caprine RBP of placental origin.

Journal of Endocrinology (1995) 146, 527–534

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Javier Blanco-Rivero, Ana Sagredo, Gloria Balfagón and Mercedes Ferrer

. 1991 , 1993 ), which is regulated by multiple kinases ( Dinerman et al. 1994 , Michell et al. 2001 ). Several studies have reported that protein kinase C (PKC) could phosphorylate eNOS and modulate its activity, thereby leading to a decrease

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Kuladip Jana, Xiangling Yin, Randolph B Schiffer, Jau-Jiin Chen, Akhilesh K Pandey, Douglas M Stocco, Paula Grammas and XingJia Wang

steroidogenic acute regulatory (StAR) protein plays a critical function at this step by facilitating the cholesterol transfer ( Clark et al . 1994 , Lin et al . 1995 , Wang et al . 1998 ). It was well demonstrated that LH stimulation of Leydig cells induces

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Hannah J Welters, Alina Oknianska, Kai S Erdmann, Gerhart U Ryffel and Noel G Morgan

, Stride & Hattersley 2002 ). Thus, identification of the proteins that are mutated in patients with MODY can provide important insights into the functional control of β-cell integrity. In this context, we have recently demonstrated that increased

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J. L. Vallet, P. J. Barker, G. E. Lamming, N. Skinner and N. S. Huskisson

ABSTRACT

Ovine trophoblast protein-1 (oTP-1), stimulates the secretion of several proteins in explant culture of day-12 cyclic ovine endometrium. We partially purified and identified one of these proteins, an 11,000 Mr, pI approx. 6 protein by N-terminal amino acid sequencing and immunoprecipitation using antibody to human β2-microglobulin. The protein was purified from cultures of endometrium collected from day-16 pregnant ewes. The N-terminal amino acid sequence was 40–55% homologous to β2-microglobulin from a variety of species. Antibody to human β2-microglobulin immunoprecipitated the protein and another protein of similar molecular weight but more acidic pi. Using immunoprecipitation of radiolabelled proteins from culture, we demonstrated that oTP-1 increased production of this protein by 40% (P<0.05). We conclude that oTP-1 increases the secretion of a β2-microglobulin-like protein from day-12 non-pregnant endometrium in culture.

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Ola Nilsson, Elizabeth A Parker, Anita Hegde, Michael Chau, Kevin M Barnes and Jeffrey Baron

identified genes whose expression levels changed dramatically during the differentiation program, including multiple genes functionally related to bone morphogenetic proteins (BMPs). BMPs belong to the transforming growth factor-β superfamily and were

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Matthias R Meyer, Natalie C Fredette, Matthias Barton and Eric R Prossnitz

including estrogen receptor α ( Green et al . 1986 , Greene et al . 1986 ) and estrogen receptor β ( Kuiper et al . 1996 ), as well as the 7-transmembrane, intracellular G protein-coupled estrogen receptor (GPER) ( Revankar et al . 2005 , Thomas et al

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Ghania Ramdani, Nadine Schall, Hema Kalyanaraman, Nisreen Wahwah, Sahar Moheize, Jenna J Lee, Robert L Sah, Alexander Pfeifer, Darren E Casteel and Renate B Pilz

et al. 2013 , Kuhn 2016 ). cGMP regulates ion channels, phosphodiesterases and two types of cGMP-dependent protein kinases (PKG1 and 2), encoded by two homologous genes ( Prkg 1 and 2) ( Hofmann et al. 2009 ). PKG1 is widely expressed with high

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Renata Lopes Araujo, Bruno Moulin de Andrade, Álvaro Souto Padron de Figueiredo, Monique Leandro da Silva, Michelle Porto Marassi, Valmara dos Santos Pereira, Eliete Bouskela and Denise P Carvalho

), which lead to thyrotoxicosis and several deleterious effects on the organism such as protein loss, heart hypertrophy, and arrhythmias ( Klein & Ojamaa 2001 ). However, the replacement of a low physiological dose of T 4 during food restriction in normal

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Esther Nuñez-Durán, Belén Chanclón, Silva Sütt, Joana Real, Hanns-Ulrich Marschall, Ingrid Wernstedt Asterholm, Emmelie Cansby and Margit Mahlapuu

metabolism, we recently identified serine/threonine protein kinase 25 (STK25, also referred to as YSK1 or SOK1), a broadly expressed member of the sterile 20 (STE20) kinase superfamily ( Thompson & Sahai 2015 ), as a central regulator of whole-body glucose