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Ljupka Gligorovska, Biljana Bursać, Sanja Kovačević, Nataša Veličković, Gordana Matić and Ana Djordjevic

-enriched diet. Glucose metabolism was assessed through analysis of systemic insulin sensitivity, while lipid metabolism was analyzed at the level of visceral adiposity, VAT histology and the expression of GC-target genes involved in lipogenesis. Besides GR

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Satoko Haeno, Naoyuki Maeda, Takeshi Yagi, Sachi Tahata, Michiko Sato, Kanako Sakaguchi, Taku Miyasho, Hiromi Ueda and Hiroshi Yokota

adrenal homogenate is also shown (C). The body and organ weights are shown in (D). The data for each group represent the mean± s.e.m . of three to four rats. * P <0.05 and ** P <0.01 compared with controls. Histological observation of the adrenal glands

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Xuefeng Yang, Shuang Mei, Haihua Gu, Huailan Guo, Longying Zha, Junwei Cai, Xuefeng Li, Zhenqi Liu and Wenhong Cao

-SOD. Histological examination of pancreas Pancreatic slices (5 mm thickness) were fixed in 10% neutral-buffered formalin/Bouin's fluid overnight. All the tissue samples were subjected to standard processing and staining procedures. Paraffin-embedded sections of 5

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Yun Wang, Yue Zheng, Patsy M Nishina and Jürgen K Naggert

, and adipose tissue inflammation B6- Nmf15 /+ mice also demonstrate both histological and pathophysiological evidence of cardiomyopathy. Histological analysis revealed that hearts from 20-week-old B6- Nmf15 /+ mice displayed distinguishable chamber

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Simone Radavelli-Bagatini, Amy R Blair, Joseph Proietto, Poli Mara Spritzer and Sofianos Andrikopoulos

.9% and interassay CV of 9.0%. Histology Ovaries were removed, fixed in Bouin's fixative, dehydrated in ethanol, and embedded in paraffin. Serial sections of 4 μm thickness were stained with hematoxylin–eosin and then evaluated for follicle stages, as

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Emily Panagiotidou, Sophia Zerva, Dimitra J Mitsiou, Michael N Alexis and Efthymia Kitraki

sections (5 μm) were cut and stained with hematoxylin and eosin to assess standard histology and morphometry. Central adrenal sections from 6 to 8 adrenals per group were digitized (Leica Microsystems GmbH, Wetzlar, Germany). We calculated the thickness of

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Cho-Rong Bae, Kazuya Hasegawa, Sayaka Akieda-Asai, Yurie Kawasaki, Kazuyo Senba, Youn-Soo Cha and Yukari Date

blotting analysis, the level of fatty acid synthase ( Fasn ) by using quantitative PCR, and the triacylglycerol content in the livers of the rats. We also carried out immunohistochemistry to evaluate the histological characteristics of pancreatic islets

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Jung Han Kim, Taryn P Stewart, Morvarid Soltani-Bejnood, Luan Wang, Jennifer M Fortuna, Ola A Mostafa, Naima Moustaid-Moussa, Ahmed M Shoieb, Michael F McEntee, Yun Wang, Lawrence Bechtel and Jürgen K Naggert

, Knoxville, TN, USA) generations were used in this study. Measurements of body weights (bw) and plasma levels of glucose, insulin, and triglyceride with age were from the F6NE4F8 and F6NE4F8+8~+10 generations. Histo-logical examination, tissue glucose uptake

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Marlise Guerrero Schimpf, María M Milesi, Enrique H Luque and Jorgelina Varayoud

centrifuged (1300  g , 20 min) and serum was stored at −20°C until hormone assay was performed. One uterine horn of each rat was fixed by immersion in 10% buffered formalin for 6 h at 4°C and processed for histology and immunohistochemistry (IHC). The other

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Rosa Chung, Bruce K Foster and Cory J Xian

histological immunostaining as well. ELISA for Bevacizumab To monitor anti-VEGF levels in rats treated with anti-VEGF, ELISA was carried out with serum of blood samples collected on days of killing (days 6 and 14). Briefly, microplate wells were coated with 100