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M van de Vyver, E Andrag, I L Cockburn and W F Ferris

Lehmann et al . (1995) and Sakamoto et al . (2000) . e Reginato et al . (1998) . Histology Lipid accumulation during both adipogenesis and osteogenic differentiation was assessed by Oil red O staining. Briefly, the media were discarded and cells were

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Hui Yu, Zoe Thompson, Sylee Kiran, Graham L Jones, Lakshmi Mundada, Surbhi, Marcelo Rubinstein and Malcolm J Low

receptor (LEPRE) was measured by ELISA (DY497, Mouse Leptin R DuoSet ELISA, R&D Systems) according to the manufacturer’s instructions. Histology Placentas were dissected at dpc18.5 and post-fixed in 10% neutral buffered formalin overnight at 4°C

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Marina Komrakova, Stephan Sehmisch, Mohammad Tezval, Ulrich Schmelz, Holm Frauendorf, Thomas Grueger, Thomas Wessling, Carolin Klein, Miriam Birth, Klaus M Stuermer and Ewa K Stuermer

tested biomechanically. One tibia, chosen randomly, was used for histological analyses. Newly formed callus at the osteotomy line of contralateral tibia was sampled for gene expression analyses after 5 weeks, the time when the callus is still forming and

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M Alexandra Sorocéanu, Dengshun Miao, Xiu-Ying Bai, Hanyi Su, David Goltzman and Andrew C Karaplis

instrument. Histology and immunohistochemistry Mice were sacrificed by cervical dislocation. The lumber vertebral bodies were removed and fixed in PLP fixative (2% paraformaldehyde containing 0.075 M lysine and 0.01 M

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Manuel C Lemos, Brian Harding, Anita A C Reed, Jeshmi Jeyabalan, Gerard V Walls, Michael R Bowl, James Sharpe, Sarah Wedden, Julie E Moss, Allyson Ross, Duncan Davidson and Rajesh V Thakker

+ /− and six Men1 −/− ). For histological examination, embryos were embedded in paraffin, and transverse sections (5–7 μm thickness) were obtained and stained with haematoxylin and eosin ( Wang et al . 2008 ). Immunostaining for menin expression was

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Jenny D Y Chow, Margaret E E Jones, Katja Prelle, Evan R Simpson and Wah Chin Boon

and weighed. Small portions of all tissues were fixed in Bouin's solution for histological examination, and the remaining was snap-frozen in liquid nitrogen then stored at −80 °C for expression studies and TG assays. Liver specimens were also embedded

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H El Sheikh Saad, A Toullec, S Vacher, M Pocard, I Bieche and M Perrot-Applanat

through graded alcohols, and paraffin embedded. Animals were killed by opening the thorax. RNA extraction Total RNA was extracted from the same individual mammary glands obtained from PND35 and PND50 rats as were used for histological studies ( El Sheikh

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Doron Bresler, Jan Bruder, Klaus Mohnike, William D Fraser and Peter S N Rowe

Brandon Sehlke for his technical assistance with the renal immuno-histology slides. We are also indebted to the support provided by the following National Institutes of Health (NIH) grants to P S N R: 1R03DE015900–01 (National Institute of Dental

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Paolo Comeglio, Ilaria Cellai, Tommaso Mello, Sandra Filippi, Elena Maneschi, Francesca Corcetto, Chiara Corno, Erica Sarchielli, Annamaria Morelli, Elena Rapizzi, Daniele Bani, Daniele Guasti, Gabriella Barbara Vannelli, Andrea Galli, Luciano Adorini, Mario Maggi and Linda Vignozzi

sensitivity in diabetics with NAFLD ( Mudaliar et al. 2013 ) and ameliorating liver histology and function in non-cirrhotic NASH patients ( Neuschwander-Tetri et al. 2015 ). Considering the pivotal role of FXR and TGR5 in metabolic control, the aim of

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João A B Pedroso, Pedro O R de Mendonca, Marco A S Fortes, Igor Tomaz, Vitor L Pecorali, Thais B Auricino, Ismael C Costa, Leandro B Lima, Isadora C Furigo, Debora N Bueno, Angela M Ramos-Lobo, Claudimara F P Lotfi and Jose Donato Jr

 = 3) were transcardiacally perfused with formalin and their brains were prepared for histological analysis, as previously described ( Furigo et al . 2014 , Nagaishi et al . 2014 ). The distribution of tdTomato-expressing cells in the brain was