then removed the kidney cortex for histological analysis and Western blotting. We calculated the mean glomerular volume by the point counting method at a magnification of 400× on hematoxylin and eosin-stained sections as described previously ( Adamczak
Jinn-Yang Chen, Deng-Yuan Jian, Chih-Chan Lien, Yu-Ting Lin, Ching-Heng Ting, Luen-Kui Chen, Ting-Chia Hsu, Hsuan-Min Huang, Yu-Ting Wu, Tse-Ting Kuan, Yu-Wen Chao, Liang-Yi Wu, Seng-Wong Huang and Chi-Chang Juan
Iwona J Bujalska, Omar M Durrani, Joseph Abbott, Claire U Onyimba, Pamela Khosla, Areeb H Moosavi, Tristan T Q Reuser, Paul M Stewart, Jeremy W Tomlinson, Elizabeth A Walker and Saaeha Rauz
for each specimen, i.e. approximately 300 adipocytes were evaluated for each histological section examined. The following descriptive variables were measured: cell diameter, length of longest line joining two points and passing through centroid
Elin Kristensson, Monika Sundqvist, Rolf Håkanson and Erik Lindström
tissue samples were collected from the acid-producing part of the stomach (fundus) for assessment of mucosal thickness (for details see Histology). In five fasted and six fed SPD rats and six fasted and fed WKY rats, the oxyntic mucosa was scraped-off the
Stephanie L Clookey, Rebecca J Welly, Terese M Zidon, Michelle L Gastecki, Makenzie L Woodford, Zachary I Grunewald, Nathan C Winn, Dusti Eaton, Natalia G Karasseva, Harold S Sacks, Jaume Padilla and Victoria J Vieira-Potter
using colorimetric ELISA (#90030, #80569, Crystal Chem) and data are presented as µg/mL. Histological assessments Formalin-fixed samples were processed through paraffin embedment, sectioned at 5 µm and stained with hematoxylin and eosin
A. C. CROOKE, A. G. DAVIES and R. MORRIS
Large doses of human chorionic gonadotrophin and human pituitary follicle-stimulating hormone were given singly and in combination to six eunuchoidal men. None had an increased excretion of urinary gonadotrophin before treatment.
Histological examination of the testicles showed very immature germinal epithelium in five of the patients before treatment. Spermatozoa were found histologically in three patients, only after combined treatment with both gonadotrophins. Low concentrations of spermatozoa appeared in semen from two of these patients.
One patient was found to have histological evidence of spermatogenesis before treatment but was unable to produce semen. Treatment with chorionic gonadotrophin alone enabled him to produce semen containing up to 15,000,000 spermatozoa per ml.
Significant increases were found in the urinary levels of a variety of steroids and in total body potassium after treatment with chorionic gonadotrophin and a variable amount of somatic development took place.
MARY F. LYON, I. HENDRY and R. V. SHORT
The effect of testosterone injections on the histology of the submaxillary salivary glands, and their content of nerve growth factor (NGF), were studied in normal and gonadectomized male and female mice, and in those affected with testicular feminization (due to the X-linked gene, Tfm). In intact and spayed female and castrated male mice testosterone increased the quantity of NGF and caused the histology of the glands to become like the male type. In mice with testicular feminization both the histology and the NGF content were of the female type, and were completely unaffected by testosterone injections. Female mice heterozygous for Tfm responded to testosterone, but in some the response appeared intermediate. The submaxillary gland provides a suitable test organ for studies of the androgen insensitivity of Tfm mice, and may prove more useful than the kidneys for some experiments.
J. D. HAHN
Adult male rats were treated with daily s.c. doses of 10 mg. cyproterone acetate for 3 weeks. Histological examination of the exorbital lacrimal glands at the end of treatment showed distinct differences compared with male controls: the histological picture showing greater resemblance to the glands of female controls. In orchidectomized rats receiving substitution treatment with testosterone propionate, cyproterone acetate 'neutralized' the effect of testosterone propionate on the exorbital lacrimal gland to a considerable extent. The possibility that this gland may play a part in the sexual behaviour of the rat is discussed.
Camila Martinez Calejman, Juan M Di Gruccio, María E Mercau, Esteban M Repetto, Francisco Astort, Rocío Sanchez, Matías Pandolfi, Gabriela Berg, Laura Schreier, Pablo Arias and Cora B Cymeryng
Laboratories). Histological and lipid analysis of adrenal glands Adrenal glands were rapidly immersed for 6 h in 4% formaldehyde diluted in 0.1 M phosphate buffer (pH 7.2), dehydrated, and then embedded in paraffin. Adrenals were sectioned (5 μm) and stained
Salla Nuutinen, Liisa Ailanen, Eriika Savontaus and Petteri Rinne
Institutes of Health) with colour deconvolution plug-in. Histological and immunohistochemical stainings The aortic roots of male mice were embedded in Tissue-Tek O.C.T. compound (Tissue-Tek, Sakura Finetek USA Inc, Torrance, CA, USA), frozen in
Flavia Fonseca Bloise, Felipe Leite de Oliveira, Alberto Félix Nobrega, Rita Vasconcellos, Aline Cordeiro, Luciana Souza de Paiva, Dennis D Taub, Radovan Borojevic, Carmen Cabanelas Pazos-Moura and Valéria de Mello-Coelho
, collection of tissues, and histological staining Bone marrow cells were obtained by flushing the femurs of the animals with culture medium (RPMI-1640, Sigma) injected through a 21 gauge needle. Spleens were removed and weighed before being smashed on a nylon