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Mary Corless, Aoife Kiely, Neville H McClenaghan, Peter R Flatt and Philip Newsholme

calcineurin activity assay kit, as described in the manufacturer’s protocol (Calbiochem). Figure 3 An electrophoretic mobility shift assay was used to confirm an increase in PDX1 transcription factor-binding activity after incubation in 10 mM l -glutamine

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B Maiztegui, M I Borelli, M A Raschia, H Del Zotto and J J Gagliardino

understand the broad mechanisms involved in β-cell adaptation to an increased demand of insulin, we studied β-cell mass, insulin secretion, glucose metabolism, and the activity, transcription, protein expression, and intracellular translocation (cellular

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Elika Missaghian, Petra Kempná, Bernhard Dick, Andrea Hirsch, Rasoul Alikhani-Koupaei, Bernard Jégou, Primus E Mullis, Brigitte M Frey and Christa E Flück

(data not shown). Also detection of CYP17 protein by western blot failed after treatment of Y1 cells with 5-aza-CdR and/or TSA. Activity of a mouse CYP17 promoter reporter construct in MA-10 and Y1 cells Several transcription factors have been identified

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Chung-Man Yeung, Chi-Bun Chan, Norman Y S Woo and Christopher H K Cheng

.aist.go.jp/research/db/TFSEARCH.html ) revealed a number of putative transcription factor-binding sites on the 5′-flanking region of the seabream ghrelin gene (Fig. 5 ). Functional mapping of the seabream ghrelin promoter The promoter activity of the 5

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A McMaster, T Chambers, Q-J Meng, S Grundy, A S I Loudon, R Donn and D W Ray

Introduction Glucocorticoid hormones exert a wide diversity of effects in target tissues. Their activity has been typically explored using a limited number of timed end points, both in vivo and in vitro , and using such approaches a variety of

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Zuzana Saidak, Carole Le Henaff, Sofia Azzi, Caroline Marty and Pierre J Marie

-related decline in bone formation results from multiple intrinsic and extrinsic mechanisms that lead to decreased differentiation of bone marrow stromal cells (BMSCs) into osteoblasts and decreased osteoblast number and activity ( Manolagas & Parfitt 2010

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L M Thurston, D R E Abayasekara and A E Michael

, luteinised human granulosa cells exhibit increased 11KSR activity ( Michael et al. 1997 , Tetsuka et al. 1997 ). The cow offers a model system in which to study changes in the expression and/or activities of the cloned 11βHSD enzymes in a

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Sarah L Alderman and Mathilakath M Vijayan

, Mommsen et al . 1999 ). Negative feedback regulation of HPI axis activity is mediated by cortisol at all levels of the axis, including suppression of CRF-mediated ACTH release ( Fryer & Peter 1977 ); however, the mechanisms governing cortisol feedback in

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V Rider, T Potapova, G Dai and M J Soares

ligated to a β-galactosidase (β-Gal) reporter gene was cotransfected with all constructs and used to correct for transfection efficiency. Kits for monitoring β-Gal activity were obtained from Tropix (Bedford, MA, USA). OPTI-MEM reduced serum medium without

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Kuladip Jana, Xiangling Yin, Randolph B Schiffer, Jau-Jiin Chen, Akhilesh K Pandey, Douglas M Stocco, Paula Grammas and XingJia Wang

 μM chrysin enhanced cAMP-induced StAR protein expression concomitantly with the increase in testosterone production ( Fig. 2 ). Chrysin-enhanced StAR gene transcription Luciferase assay of StAR promoter activity and RT-PCR analysis of StAR mRNA levels