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Gina L C Yosten, Grant R Kolar, Lauren J Redlinger and Willis K Samson

-associated microvascular dysfunction is complex and poorly understood, and thus, therapeutic interventions to prevent or treat these disorders are limited. However, proinsulin C-peptide has emerged as a promising target for the treatment of diabetes

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Mahendra Prasad Bhatt, Yeon-Ju Lee, Se-Hui Jung, Yong Ho Kim, Jong Yun Hwang, Eun-Taek Han, Won Sun Park, Seok-Ho Hong, Young-Myeong Kim and Kwon-Soo Ha

preventing long-term cardiovascular complications associated with diabetes ( Ihnat et al . 2007 , Ceriello et al . 2009 a , b ). C-peptide is one such potential agent, due to its ability to inhibit ROS-mediated intracellular events, including mitochondrial

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Jia-Fwu Shyu, Hwai-Shi Wang, Yi-Ming Shyr, Shin-E Wang, Chia-Hsiang Chen, Joo-Shin Tan, Meng-Feng Lin, Po-Shiuan Hsieh, Huey-Kang Sytwu and Tien-Hua Chen

pancreatitis 3 (5.3) 1 (5.0) 2 (5.4)  Others 11 (19.3) 5 (25.0) 6 (16.2) * P <0.05. C, using the χ 2 test; F, using Fisher's exact test. a Success was determined by formation of islet-like cell cluster and expression of PDX1 and C-peptide. The human

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L Bai, G Meredith and B E Tuch

-ethanol at 4 °C. Immunoreactive insulin content was measured by RIA using a rat insulin standard; the lower limit of sensitivity of the assay was 0.25 ng/ml. Cross-reactivity with proinsulin was 73%. C-peptide To measure C-peptide

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Xinrong Zhou, Bangguo Qian, Ning Ji, Conghui Lui, Zhiyuan Liu, Bing Li, Huarong Zhou and Caifeng Yan

plasma c-peptide, glucagon like peptide-1, and gastric inhibitory polypeptide Rats were fasted overnight before OGTT. Glucose load (2 g/kg body weight) was administered orally. Glucose levels were measured from tail bleed with a glucometer (Roche) at 0

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Jian-Ting Ke, Mi Li, Shi-Qing Xu, Wen-Jian Zhang, Yong-Wei Jiang, Lan-yun Cheng, Li Chen, Jin-Ning Lou and Wei Wu

, St Charles, MO, USA) were used to measure urinary protein and urinary albumin levels. Detection of serum C-peptide After 4 weeks of treatment, animals were killed with 2.5% pentobarbital sodium (i.p.) and serum was isolated from venous blood. A rat C-peptide

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Kaiyuan Yang, Jonathan Gotzmann, Sharee Kuny, Hui Huang, Yves Sauvé and Catherine B Chan

ImageJ (NIH, Bethesda, MD, USA), as described previously ( Whitlock et al. 2012 ). The staining intensity of insulin-, proinsulin-, and C-peptide-positive areas was similarly measured. For immunofluorescent staining, slides were mounted with ProLong

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Masaki Nagaya, Hitoshi Katsuta, Hideaki Kaneto, Susan Bonner-Weir and Gordon C Weir

19; a gift of Dr A Miyajima, Tokyo University, Japan), rabbit anti-NeuroD (1:200; Cell Signaling Technology, Inc., Danvers, MA, USA), rabbit anti-Pdx-1 (1:2000; IPF-1C antibody, a gift from Dr J M W Slack, University of Minnesota) and rabbit anti-C-peptide

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Franka S Schaebs, Tanja E Wolf, Verena Behringer and Tobias Deschner

intensive observation times and data collection. An alternative is the quantification of biomarkers of energy balance in noninvasively collected samples. Recent studies have validated the use of urinary C-peptide (UCP) as a physiological marker to assess

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Esther Nuñez-Durán, Belén Chanclón, Silva Sütt, Joana Real, Hanns-Ulrich Marschall, Ingrid Wernstedt Asterholm, Emmelie Cansby and Margit Mahlapuu

control, 18S rRNA (Applied Biosystems). Biochemical assays The fasting plasma insulin, C-peptide, leptin and glucagon levels were assessed using the Ultrasensitive Mouse Insulin ELISA kit (Chrystal Chem Inc., Downers Grove, IL, USA), the Mouse C-Peptide