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Stina Häggström Rudolfsson and Anders Bergh

of hypoxia-inducible factor 1α (HIF-1α) and this is the major stimulator of VEGF, and other angiogenesis stimulators, secretion in various tissues ( Dor et al . 2001 , Giordano & Johnson 2001 , Semensa 2003 ). It is therefore somewhat surprising

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Laura E Pascal, Khalid Z Masoodi, June Liu, Xiaonan Qiu, Qiong Song, Yujuan Wang, Yachen Zang, Tiejun Yang, Yao Wang, Lora H Rigatti, Uma Chandran, Leandro M Colli, Ricardo Z N Vencio, Yi Lu, Jian Zhang and Zhou Wang

-deoxycholate, 150 mm NaCl, 1 mm EDTA (pH 8.0), 1 mm NaF, 2 mm phenylmethylsulfonylfluoride, 1 mm Na 3 VO 4 , and protease inhibitor cocktail (Sigma)). Western blot analysis of ELL2, HIF1α and GAPDH was as described previously ( Saporita et al. 2007 ). Antibodies

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Masami Hayashi, Masahiro Sakata, Takashi Takeda, Toshiya Yamamoto, Yoko Okamoto, Kenjiro Sawada, Akiko Kimura, Ryoko Minekawa, Masahiro Tahara, Keiichi Tasaka and Yuji Murata

the changes of expression of several genes in response to the cellular oxygen concentration ( Semenza & Wang 1992 ). HIF-1 consists of HIF-1α and HIF-1β subunits, both of which belong to the basic-loop-helix Per-Arnt-Sim (PAS) protein family ( Wang et

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Toshiaki Ishizuka, Takashi Hinata and Yasuhiro Watanabe

hypoxia. HIF-1α, α subunit of HIF-1, is hydroxylated under normoxia but is stabilized under hypoxia. HIF-1α regulates the transcription of VEGF, platelet-derived growth factor (PDGF), and FGF-2 in response to hypoxia ( Nilsson et al . 2004 , Calvani et

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Daisuke Fujita, Akiko Tanabe, Tatsuharu Sekijima, Hekiko Soen, Keijirou Narahara, Yoshiki Yamashita, Yoshito Terai, Hideki Kamegai and Masahide Ohmichi

-AKT polyclonal antibodies, anti-phosphorylated AKT polyclonal antibodies, and anti-phosphorylated MTOR antibodies were obtained from Cell Signaling Technology, Inc. (Danvers, MA, USA). The anti-HIF-1α monoclonal and anti-NFκB antibodies were obtained from Becton

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Elisabete A Forsberg, Ileana R Botusan, Jing Wang, Verena Peters, Ishrath Ansurudeen, Kerstin Brismar and Sergiu Bogdan Catrina

two subunits α and β, in which the α subunit is regulated by oxygen. In the presence of oxygen, HIF is hydroxylated by a specific Fe 2 + , oxoglutarate dependent prolyl 4-hydroxylases (PHD) allowing HIF-1α to bind to the von Hippel-Lindau (VHL) tumor

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Yan Lin, Suyi Li, Peng Cao, Lu Cheng, Ming Quan and Suyu Jiang

-PCR The RNA expression of Ghr, Jak-2, Stat3, Vegf, Hif-1α, Fgf , and Mmp-2 was measured by RT-PCR. Total RNA was extracted from tissues using Trizol (Invitrogen) according to the manufacturer's protocol. Primer sequences (forward/reverse) and RT

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Bohan Wang, I Stuart Wood and Paul Trayhurn

PCR efficiency in all runs was close to 100%, and all samples were analyzed in at least duplicate. Measurement of HIF-1α by western blotting and ELISA Cells were solubilized in lysis buffer consisting of 50 mM Tris (pH 7.4), 300 mM NaCl, 10% (w

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Hsiu-Chi Lee and Shaw-Jenq Tsai

subunit, also known as aryl hydrocarbon receptor nuclear translocator and an oxygen-sensitive α subunit. There are three members of HIF-α in mammal ( Fig. 1 ). HIF-1α and HIF-2α proteins are critical for mediating the adaptive response of hypoxia, whereas

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Alpha Dian-Yu Lin, Anita Mannikarottu, Barry A Kogan, Catherine Whitbeck, Paul Chichester, Robert E Leggett and Robert M Levin

hypertrophy. In addition, we investigated the expression of two highly potent angiogenic markers, vascular endothelial growth factor (VEGF) and hypoxia inducible factor (HIF-1α) in the hypertrophied bladder. Materials and Methods