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Introduction Monocyte chemoattractant protein-1 (MCP-1, CCL2) acts as a chemoattractant for monocytes and memory T cells via the binding of its receptor, CC-chemokine receptor-2 (CCR2). CCR2 belongs to the G-protein-coupled seven
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infiltrated macrophages in visceral adipose tissue through various inflammatory molecules including tumor necrosis factor-α (TNF-α; Hotamisligil 2006 ), interleukin-6 (IL-6; Fernandez-Real & Ricart 2003 ), and monocyte chemoattractant protein-1 (MCP-1
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factor β (TGFβ1), monocyte chemoattractant protein 1 (MCP-1), and plasminogen activator inhibitor-1 (PAI-1; Tilg & Moschen 2006 ). TGFβ1 reduces serum-stimulated proliferation of bovine mammary epithelial cells ( Woodward et al . 1995 , Purup et al
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plasma levels of monocyte chemoattractant protein-1 (MCP-1) at 9 weeks of age and retinol binding protein-4 (RBP4) and soluble intercellular adhesion molecule-1 (sICAM 1) at 12 weeks of age. At 14–16 weeks of age, insulin sensitivity was measured with a
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Departments of, Cardiovascular Medicine, Advanced Therapeutics for Cardiovascular Diseases, Kyushu University Graduate School of Medical Sciences, Fukuoka, Japan
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Departments of, Cardiovascular Medicine, Advanced Therapeutics for Cardiovascular Diseases, Kyushu University Graduate School of Medical Sciences, Fukuoka, Japan
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, which is one of the causes of insulin resistance ( Savage et al . 2007 ). The hypertrophied adipocytes produce proinflammatory cytokines such as monocyte chemoattractant protein-1 (MCP1 (CCL2)) and tumor necrosis factor-α (TNF-α) as obesity progresses
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disease in humans ( Kern et al . 2001 , Pradhan et al . 2001 , Mertens & Van Gaal 2002 , Spranger et al . 2003 ). The pro-inflammatory monocyte chemoattractant protein-1 (MCP1 or CCL2 as listed in the MGI Database) which is elevated in obesity
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adipocytes, cells were harvested and mRNA was isolated applying RNeasy Mini Kit according to manufacturer’s recommendations. Quantification of adiponectin, GLUT1, GLUT4, IL-6, MCP-1 and TLR9 mRNA expression in murine 3T3-L1 adipocytes in vitro and ex
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Key Laboratory of Metabolism of Lipid and Glucose, John Moorhead Research Laboratory, Centre for Lipid Research, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, China
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Bio-Rad Mini Protean apparatus and then transferred to a PVDF membrane. The membrane was blocked with 5% (w/v) non-fat dried milk and incubated with primary antibodies (anti-TNFα, anti-MCP1, anti-insulin receptor substrate 1 (IRS1), anti-IRS2, anti
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Institute of Health Research-INCLIVA, Endocrinology and Nutrition Department Clinic Hospital and Department of Medicine, CIBER de Diabetes y Enfermedades Metabólicas asociadas (CIBERDEM), Department of Farmacology, Centro de Investigación Príncipe Felipe, Avenida Menéndez Pelayo, 4, 46010 Valencia, Spain
Institute of Health Research-INCLIVA, Endocrinology and Nutrition Department Clinic Hospital and Department of Medicine, CIBER de Diabetes y Enfermedades Metabólicas asociadas (CIBERDEM), Department of Farmacology, Centro de Investigación Príncipe Felipe, Avenida Menéndez Pelayo, 4, 46010 Valencia, Spain
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Institute of Health Research-INCLIVA, Endocrinology and Nutrition Department Clinic Hospital and Department of Medicine, CIBER de Diabetes y Enfermedades Metabólicas asociadas (CIBERDEM), Department of Farmacology, Centro de Investigación Príncipe Felipe, Avenida Menéndez Pelayo, 4, 46010 Valencia, Spain
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Institute of Health Research-INCLIVA, Endocrinology and Nutrition Department Clinic Hospital and Department of Medicine, CIBER de Diabetes y Enfermedades Metabólicas asociadas (CIBERDEM), Department of Farmacology, Centro de Investigación Príncipe Felipe, Avenida Menéndez Pelayo, 4, 46010 Valencia, Spain
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Institute of Health Research-INCLIVA, Endocrinology and Nutrition Department Clinic Hospital and Department of Medicine, CIBER de Diabetes y Enfermedades Metabólicas asociadas (CIBERDEM), Department of Farmacology, Centro de Investigación Príncipe Felipe, Avenida Menéndez Pelayo, 4, 46010 Valencia, Spain
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, Samuel & Shulman 2012 ). Moreover, excess fat in adipocytes, which occurs in overweight and obesity, aggravates fatty liver disease by increasing the release of pro-inflammatory mediators such as MCP1, IL-6 and TNFα ( Sahini & Borlak 2014 ). Of note, one
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described in Materials and Methods. A bar graph analysis of four independent experiments is shown. * P <0.05, ** P <0.01. By contrast, pro-inflammatory adipokines monocyte chemoattractant protein-1 (MCP-1) and IL-6 were up-regulated by ACTH treatment. MCP-1