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Juliano C da Silveira, Ana Clara F C M de Ávila, Hannah L Garrett, Jason E Bruemmer, Quinton A Winger and Gerrit J Bouma

Introduction Proper cell function requires the coordinated regulation of a myriad of genes and proteins necessary for maintenance of tissue homeostasis and organism fitness. MicroRNAs are small non-coding RNA molecules that regulate gene

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Niels L Mulder, Rick Havinga, Joost Kluiver, Albert K Groen and Janine K Kruit

cellular miRNAs transcripts due to the same processing pathways ( Grimm et al. 2006 ). Therefore, we tested the expression of miR-375, a microRNA highly expressed in beta cells and involved in regulation of insulin secretion ( Poy et al. 2004 ) and miR

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Trinna L Cuellar and Michael T McManus

Introduction A whole new class of molecules has been discovered that potently regulate gene expression. These molecules are non-protein-coding small RNAs aptly referred to as microRNAs (miRNAs), as they are approximately 21

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Haiyong Chen, Hui-Yao Lan, Dimitrios H Roukos and William C Cho

) ( Fowler 2008 ). Though conventional treatments for diabetes are effective, recent advances in molecular biology have provided a better understanding of diabetes and the potential to develop molecular theranostics for the disease. MicroRNAs (miRNAs) play a

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Charlotte Benoit, Hassina Ould-Hamouda, Delphine Crepin, Arieh Gertler, Laurence Amar and Mohammed Taouis

, posttranscriptional events such as RNA stabilization are important for cell differentiation. MicroRNAs (miRNAs) have emerged as powerful regulators acting at the posttranscriptional level implicated in a wide range of cellular functions. miRNAs are small endogenous

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Omkaram Gangisetty, Shaima Jabbar, Olivia Wynne and Dipak K Sarkar

12 h of transfection, cells were treated with 50 mM ethanol at 12-h intervals for a period of 24 h. Treated cells were extracted for miRs and total RNA using mirVana microRNA isolation kit (Life Technologies) and RNeasy kit (Qiagen) and determined the

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Eric W-F Lam, Kunal Shah and Jan J Brosens

Introduction Micro-RNAs (miRNAs) are small non-coding RNAs, ∼22 nucleotides in length, which function as posttranscriptional regulators of gene expression. To date, almost 1000 human miRNAs have been identified and validated ( Griffiths-Jones 2010

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Zerui Wu, Lin Cai, Jianglong Lu, Chengde Wang, Jiaqing Guan, Xianbin Chen, Jinsen Wu, Weiming Zheng, Zhebao Wu, Qun Li and Zhipeng Su

Zhang WC Slack FJ 2017 Targeting noncoding RNAs in disease . Journal of Clinical Investigation 127 761 – 771 . ( ) 10.1172/JCI84424 Ahmad N Haider S Jagannathan S Anaissie E Driscoll JJ 2014 MicroRNA

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Ewa Ocłoń, Anna Latacz, Joanna Zubel–Łojek and Krystyna Pierzchała–Koziec

) by quantitative RT-PCR was performed using total RNA from the 24 porcine samples studied and microRNA-specific primers (TaqMan MicroRNA Assay, Life Technologies). Briefly, reverse transcription (TaqMan MicroRNA Reverse Transcription Kit, Life

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Kimberley C W Wang, Kimberley J Botting, Song Zhang, I Caroline McMillen, Doug A Brooks and Janna L Morrison

moderated by epigenetic mechanisms, such as DNA methylation, histone acetylation and microRNA (miR), which will be discussed later in this review. Furthermore, the activation of both of these signaling pathways can regulate the activity of downstream