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Rachel A Davey, Michele V Clarke, Suzanne B Golub, Patricia K Russell, and Jeffrey D Zajac

embedded within the bone matrix and constituting the most abundant cell type within bone, are also able to mediate calcium flux from bone by dissolution of the peri-lacunar matrix (osteocytic osteolysis) ( Atkins & Findlay 2012 , Qing et al. 2012 ). The

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Miskal Sbaihi, Karine Rousseau, Sylvie Baloche, François Meunier, Martine Fouchereau-Peron, and Sylvie Dufour

.001 as compared with controls. Effect of cortisol on periosteocytic osteolysis Microradiographs of ultrathin vertebral sections (20 μm; Fig. 4 A) revealed that the osteocytic lacunae observed in cortisol-treated eels were larger than those in control

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J. P. WHALEN, L. KROOK, I. MACINTYRE, and E. A. NUNEZ

SUMMARY

After calcitonin injection, parathyroidectomy, or both, in young growing rats, broadening of the proximal femoral metaphysis with lack of normal concavity of the medial and lateral contours was observed radiographically. Histologically, this abnormal modelling was associated with retarded osteocytic osteolysis. Further manifestations of decelerated resorption included retention and extension of the chondroid core in the secondary spongiosa, retention of cartilage in the cortex and a large increase in the number of cementing lines.

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M Jevon, A Sabokbar, Y Fujikawa, T Hirayama, SD Neale, J Wass, and NA Athanasou

A number of bone diseases characterised by excessive osteolysis (e.g. osteoporosis and Paget's disease) exhibit a marked gender difference in prevalence and are more common in the elderly population. Bone resorption is carried out by osteoclasts, which are formed by fusion of circulating mononuclear precursor cells of haematopoietic origin. In this study, we have determined whether there are gender- and age-related differences in osteoclast formation from circulating precursors. Peripheral blood mononuclear cells (PBMCs) were co-cultured with UMR106 osteoblast-like cells in the presence of macrophage-colony stimulating factor (M-CSF) and 1,25 dihydroxyvitamin D(3) (1,25(OH)(2)D(3)) or cultured alone in the presence of sRANKL (soluble receptor activator of nuclear factor kappa B ligand) and M-CSF. As assessed by the formation of tartrate resistant acid phosphatase (TRAP)-positive (TRAP(+)) and vitronectin receptor-positive (VNR(+)) multinucleated cells (MNCs), there was no difference in the number of circulating osteoclast precursors in males and females. Lacunar resorption carried out by osteoclasts formed from these precursors was generally increased in males compared with females (P=0.03). An increase in the number of TRAP(+) and VNR(+) MNCs formed from male PBMCs was noted in response to 1,25(OH)(2)D(3) (P<0.005). An increase in lacunar resorption in cultures of PBMCs (10(5) per well) from males was also noted in response to 10(-9) M 1,25(OH)(2)D(3) (P<0.05) and sRANKL (P=0.05), but not M-CSF. The addition of dexamethasone resulted in a marked increase in osteoclast formation and lacunar resorption in both males and females. Post-menopausal females and males of comparable age showed similar levels of osteoclastogenesis. Pre-menopausal women showed similar levels of osteoclastogenesis but less resorption (P=0.01) compared with males of comparable age. These results show that there are specific gender/age-related differences in osteoclast formation and bone resorption and have implications for evaluating osteoclastogenesis in skeletal diseases such as primary osteoporosis and Paget's disease.

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Masaki Nakano, Mika Ikegame, Junko Igarashi-Migitaka, Yusuke Maruyama, Nobuo Suzuki, and Atsuhiko Hattori

to be involved in osteocytic osteolysis. However, since there was no correlation between mRNA expression levels of cathepsin K and sclerostin, an osteocyte marker ( r  = 0.053), we inferred that these factors were expressed principally by osteoclasts