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Sara Della Torre, Gianpaolo Rando, Clara Meda, Paolo Ciana, Luisa Ottobrini and Adriana Maggi

active in most reproductive and non-reproductive tissue cells ( Ciocca & Roig 1995 , Maggi et al. 2004 , Bookout et al. 2006 ). Their expression and transcriptional activity in the course of embryo development is less studied ( Brandenberger et al

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Chiung-Zuan Chiu, Bao-Wei Wang and Kou-Gi Shyu

under normoxia also increased UII expression ( Fig. 4 B and C). Hypoxia increases the binding between the UII promoter and AP1 transcription factor and enhances genetic transcription activity of AP1 to UII promoter in cardiomyocytes Under 2.5% O 2

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Miroslav Adzic, Jelena Djordjevic, Ana Djordjevic, Ana Niciforovic, Constantinos Demonacos, Marija Radojcic and Marija Krstic-Demonacos

-releasing hormone (CRH), brain-derived neurotropic factor (BDNF), and cytokines ( Goujon et al . 1997 , Schulkin et al . 1998 , Morsink et al . 2006 , Schulte-Herbruggen et al . 2006 ). The GR transcriptional activity is dependent on the cell type, the

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CG Korkmaz, K Fronsdal, Y Zhang, PI Lorenzo and F Saatcioglu

Androgens are critical in the development and maintenance of the male reproductive system and important in the progression of prostate cancer. The effects of androgens are mediated by the androgen receptor (AR), which is a ligand-modulated transcription factor that belongs to the nuclear receptor superfamily. We and others have previously shown that CREB-binding protein (CBP) can function as a coactivator for AR. Similar to some other nuclear receptor coactivators and/or the proteins that they interact with, CBP has histone acetyl transferase (HAT) activity that is thought to contribute to transcriptional activation by nuclear receptors. We have therefore assessed whether an increase in the histone acetylation status in the cell can influence AR transcriptional activity, by using the histone deacetylase (HDAC) inhibitors (HDACIs) trichostatin A (TSA), sodium butyrate (Na-But) and depsipeptide (FR901228). We found that inhibition of HDAC activity significantly increased the ability of endogenous AR in LNCaP cells, or ectopically expressed AR in HeLa cells, to activate transcription from AR-dependent reporter constructs. In addition, HDACIs increased the androgen-dependent activation of the prostate-specific antigen (PSA) gene in LNCaP cells, an increase that was not due to an increase in nuclear AR protein levels. Moreover, the viral oncoprotein E1A that inhibits CBP HAT activity fully repressed the ability of HDACIs to stimulate AR-mediated transcription, indicating that CBP is involved in this process. Deletional mutagenesis of AR indicated that whereas the AF-2 domain in the C-terminus is dispensable, the AF-1 domain in the N-terminus is required for augmentation of AR action by HDACIs, an observation which is in concordance with the reduced ability of CBP to activate AR N-terminal deletion mutants. Furthermore, HDACI treatment rescued the deficiency in the transactivation potential of AF-2 mutants. Taken together, our findings suggest that a change in the level of histone acetylation of target genes is an important determinant of AR action, possibly mediated by CBP.

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Eliane Correa-de-Santana, Bianca Fröhlich, Marta Labeur, Marcelo Páez-Pereda, Marily Theodoropoulou, Jose Luis Monteserin, Ulrich Renner and Günter K Stalla

each other ( Battle & Frank 2002 ). NFKB p65 homodimers bind to phosphothyrosine STAT3 monomers. This interaction appears to increase transcriptional activity of genes driven by specific κB elements ( Yoshida et al . 2004 ). Moreover, inhibition of

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Mei-Fway Iu, Hiroshi Kaji, Hideaki Sowa, Junko Naito, Toshitsugu Sugimoto and Kazuo Chihara

on the transcriptional activity induced by Smad3 In order to examine the mechanism by which Dex suppresses TGF-β–Smad3 pathways, we investigated whether Dex affects the transcriptional activity induced by TGF-β as well as Smad3 in MC3T3-E1

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Lisa L Koorneef, Jan Kroon, Eva M G Viho, Lucas F Wahl, Kim M L Heckmans, Marloes M A R van Dorst, Menno Hoekstra, René Houtman, Hazel Hunt and Onno C Meijer

the complex can bind to the DNA to induce or repress gene expression, generally involving parallel regulation of hundreds of genes. Eventual GR transcriptional activity is influenced by ligand concentration, receptor levels and the presence of

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Jinwon Eo, Kyuyong Han, Kenneth M Murphy, Haengseok Song and Hyunjung Jade Lim

factors increases transcriptional activity of the mPtgs2 promoter. As shown in Fig. 5 B, the addition of Etv5 increases basal activity of the 3.2 kb mPtgs2 promoter close to 2.5-fold. Etv1 did not have such effect. As shown in Fig. 5 C and as

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Melyssa R Bratton, James W Antoon, Bich N Duong, Daniel E Frigo, Syreeta Tilghman, Bridgette M Collins-Burow, Steven Elliott, Yan Tang, Lilia I Melnik, Ling Lai, Jawed Alam, Barbara S Beckman, Steven M Hill, Brian G Rowan, John A McLachlan and Matthew E Burow

. Dopamine has been shown to activate ER-mediated transcriptional activity in the absence ( Power et al . 1991 ) or presence ( Smith et al . 1993 ) of E 2 . Melatonin has been shown to inhibit breast cancer cell proliferation through modulation of ERα

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Yeon Jean Cho, Jiyeun E Lee, Mi Jin Park, Bert W O’Malley and Sang Jun Han

indicated expression plasmids. For the determination of ERβ transcriptional activity, estradiol (10 −8  M) was added to cells 24 h following transfection and incubated for another 24 h. The cells were harvested, and the luciferase activity was determined and