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Laura E Pascal, Khalid Z Masoodi, June Liu, Xiaonan Qiu, Qiong Song, Yujuan Wang, Yachen Zang, Tiejun Yang, Yao Wang, Lora H Rigatti, Uma Chandran, Leandro M Colli, Ricardo Z N Vencio, Yi Lu, Jian Zhang and Zhou Wang

-deoxycholate, 150 mm NaCl, 1 mm EDTA (pH 8.0), 1 mm NaF, 2 mm phenylmethylsulfonylfluoride, 1 mm Na 3 VO 4 , and protease inhibitor cocktail (Sigma)). Western blot analysis of ELL2, HIF1α and GAPDH was as described previously ( Saporita et al. 2007 ). Antibodies

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Stina Häggström Rudolfsson and Anders Bergh

of hypoxia-inducible factor 1α (HIF-1α) and this is the major stimulator of VEGF, and other angiogenesis stimulators, secretion in various tissues ( Dor et al . 2001 , Giordano & Johnson 2001 , Semensa 2003 ). It is therefore somewhat surprising

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Masami Hayashi, Masahiro Sakata, Takashi Takeda, Toshiya Yamamoto, Yoko Okamoto, Kenjiro Sawada, Akiko Kimura, Ryoko Minekawa, Masahiro Tahara, Keiichi Tasaka and Yuji Murata

the changes of expression of several genes in response to the cellular oxygen concentration ( Semenza & Wang 1992 ). HIF-1 consists of HIF-1α and HIF-1β subunits, both of which belong to the basic-loop-helix Per-Arnt-Sim (PAS) protein family ( Wang et

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Toshiaki Ishizuka, Takashi Hinata and Yasuhiro Watanabe

hypoxia. HIF-1α, α subunit of HIF-1, is hydroxylated under normoxia but is stabilized under hypoxia. HIF-1α regulates the transcription of VEGF, platelet-derived growth factor (PDGF), and FGF-2 in response to hypoxia ( Nilsson et al . 2004 , Calvani et

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Daisuke Fujita, Akiko Tanabe, Tatsuharu Sekijima, Hekiko Soen, Keijirou Narahara, Yoshiki Yamashita, Yoshito Terai, Hideki Kamegai and Masahide Ohmichi

)-1α (listed as HIF1A in the Hugo Database) activation, which results in endothelial cell proliferation and tube formation ( Yamakawa et al . 2003 ). VEGF has been implicated as playing an important role in placental angiogenesis ( Ferrara et al

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B Shan, C Schaaf, A Schmidt, K Lucia, M Buchfelder, M Losa, D Kuhlen, J Kreutzer, M J Perone, E Arzt, G K Stalla and U Renner

oxygen and nutrients to the tumour cells and to remove intratumoural waste products ( Turner et al . 2000 , Carmeliet 2003 ). Tumour neovascularisation is induced by intratumoural hypoxia, which leads to an increase in hypoxia inducible factor 1α (HIF1A

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Elisabete A Forsberg, Ileana R Botusan, Jing Wang, Verena Peters, Ishrath Ansurudeen, Kerstin Brismar and Sergiu Bogdan Catrina

two subunits α and β, in which the α subunit is regulated by oxygen. In the presence of oxygen, HIF is hydroxylated by a specific Fe 2 + , oxoglutarate dependent prolyl 4-hydroxylases (PHD) allowing HIF-1α to bind to the von Hippel-Lindau (VHL) tumor

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Bohan Wang, I Stuart Wood and Paul Trayhurn

PCR efficiency in all runs was close to 100%, and all samples were analyzed in at least duplicate. Measurement of HIF-1α by western blotting and ELISA Cells were solubilized in lysis buffer consisting of 50 mM Tris (pH 7.4), 300 mM NaCl, 10% (w

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Yan Lin, Suyi Li, Peng Cao, Lu Cheng, Ming Quan and Suyu Jiang

, China); signal transducers and activators of transcription 3 (STAT3), phosphorylated STAT3 (pSTAT3), hypoxia-inducible factor 1, alpha subunit (HIF-α), matrix metalloproteinase 2 (MMP-2), and other antibodies were purchased from Santa Cruz Co., (Santa

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Ángela Sánchez, Constanza Contreras-Jurado, Diego Rodríguez, Javier Regadera, Susana Alemany and Ana Aranda

) spleens. Data are means ±  s.e. (C) Transcript levels for the erythopoietic transcription factors Klf9 , Gata1 , Hif1 α and Hif2α in the spleen of Wt ( n  = 4) and KO ( n  = 6) mice. Data are expressed relative to the levels obtained in Wt mice