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Emin Umit Bagriacik Immunology Research Center, Department of Immunology, Department of Hematology, Endocrinology Department, Gazi University, 06500 Ankara, Turkey
Immunology Research Center, Department of Immunology, Department of Hematology, Endocrinology Department, Gazi University, 06500 Ankara, Turkey

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Melek Yaman Immunology Research Center, Department of Immunology, Department of Hematology, Endocrinology Department, Gazi University, 06500 Ankara, Turkey

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Rauf Haznedar Immunology Research Center, Department of Immunology, Department of Hematology, Endocrinology Department, Gazi University, 06500 Ankara, Turkey

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Gulsan Sucak Immunology Research Center, Department of Immunology, Department of Hematology, Endocrinology Department, Gazi University, 06500 Ankara, Turkey

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Tuncay Delibasi Immunology Research Center, Department of Immunology, Department of Hematology, Endocrinology Department, Gazi University, 06500 Ankara, Turkey

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extrathyroidal tissues has not been clarified. Human bone marrow-derived mesenchymal stem cells (hBMSCs) have been characterized by many properties so far. They are pluripotent cells that are capable of differentiating into a variety of mesenchymal tissues

Article Type:
Research Article
DOI:
https://doi.org/10.1530/JOE-11-0404
Volume/Issue:
Volume 212: Issue 2
Free access
Ana María Pino Laboratorio de Biología Celular, INTA, Universidad de Chile, El Líbano 5524, Macul. Casilla 138-11, Santiago, Chile
Laboratorio de Envejecimiento y Enfermedades Crónicas Relacionadas con la Nutrición, INTA, Universidad de Chile, Santiago, Chile
Servicio de Traumatología, Hospital Sótero del Río, Santiago, Chile

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Juan Manuel Rodríguez Laboratorio de Biología Celular, INTA, Universidad de Chile, El Líbano 5524, Macul. Casilla 138-11, Santiago, Chile
Laboratorio de Envejecimiento y Enfermedades Crónicas Relacionadas con la Nutrición, INTA, Universidad de Chile, Santiago, Chile
Servicio de Traumatología, Hospital Sótero del Río, Santiago, Chile

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Susana Ríos Laboratorio de Biología Celular, INTA, Universidad de Chile, El Líbano 5524, Macul. Casilla 138-11, Santiago, Chile
Laboratorio de Envejecimiento y Enfermedades Crónicas Relacionadas con la Nutrición, INTA, Universidad de Chile, Santiago, Chile
Servicio de Traumatología, Hospital Sótero del Río, Santiago, Chile

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Pablo Astudillo Laboratorio de Biología Celular, INTA, Universidad de Chile, El Líbano 5524, Macul. Casilla 138-11, Santiago, Chile
Laboratorio de Envejecimiento y Enfermedades Crónicas Relacionadas con la Nutrición, INTA, Universidad de Chile, Santiago, Chile
Servicio de Traumatología, Hospital Sótero del Río, Santiago, Chile

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Laura Leiva Laboratorio de Biología Celular, INTA, Universidad de Chile, El Líbano 5524, Macul. Casilla 138-11, Santiago, Chile
Laboratorio de Envejecimiento y Enfermedades Crónicas Relacionadas con la Nutrición, INTA, Universidad de Chile, Santiago, Chile
Servicio de Traumatología, Hospital Sótero del Río, Santiago, Chile

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Germán Seitz Laboratorio de Biología Celular, INTA, Universidad de Chile, El Líbano 5524, Macul. Casilla 138-11, Santiago, Chile
Laboratorio de Envejecimiento y Enfermedades Crónicas Relacionadas con la Nutrición, INTA, Universidad de Chile, Santiago, Chile
Servicio de Traumatología, Hospital Sótero del Río, Santiago, Chile

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Mireya Fernández Laboratorio de Biología Celular, INTA, Universidad de Chile, El Líbano 5524, Macul. Casilla 138-11, Santiago, Chile
Laboratorio de Envejecimiento y Enfermedades Crónicas Relacionadas con la Nutrición, INTA, Universidad de Chile, Santiago, Chile
Servicio de Traumatología, Hospital Sótero del Río, Santiago, Chile

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J Pablo Rodríguez Laboratorio de Biología Celular, INTA, Universidad de Chile, El Líbano 5524, Macul. Casilla 138-11, Santiago, Chile
Laboratorio de Envejecimiento y Enfermedades Crónicas Relacionadas con la Nutrición, INTA, Universidad de Chile, Santiago, Chile
Servicio de Traumatología, Hospital Sótero del Río, Santiago, Chile

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Introduction Human bone marrow stroma contains mesenchymal stem cells (hMSCs) differentiating along osteogenic, chondrogenic, adipogenic and marrow stromal lineages ( Caplan 1991 , Bruder et al. 1997 , Pittenger et al. 1999

Article Type:
Research Article
DOI:
https://doi.org/10.1677/joe.1.07026
Volume/Issue:
Volume 191: Issue 3
Free access
Noriko Sakai
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Hiromi Terami
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Shinobu Suzuki
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Megumi Haga
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Ken Nomoto
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Nobuko Tsuchida
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Ken-ichirou Morohashi Department of Stem Cell Based Drug Discovery, Division of Sex Differentiation, Laboratory of Molecular Pharmacology, Department of Systems Biomedicine, Research Center Kobe, Bayer Yakuhin Ltd, Kobe, Hyogo 650-0047, Japan

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Naoaki Saito Department of Stem Cell Based Drug Discovery, Division of Sex Differentiation, Laboratory of Molecular Pharmacology, Department of Systems Biomedicine, Research Center Kobe, Bayer Yakuhin Ltd, Kobe, Hyogo 650-0047, Japan

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Maki Asada Department of Stem Cell Based Drug Discovery, Division of Sex Differentiation, Laboratory of Molecular Pharmacology, Department of Systems Biomedicine, Research Center Kobe, Bayer Yakuhin Ltd, Kobe, Hyogo 650-0047, Japan

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Megumi Hashimoto Department of Stem Cell Based Drug Discovery, Division of Sex Differentiation, Laboratory of Molecular Pharmacology, Department of Systems Biomedicine, Research Center Kobe, Bayer Yakuhin Ltd, Kobe, Hyogo 650-0047, Japan

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Daisuke Harada Department of Stem Cell Based Drug Discovery, Division of Sex Differentiation, Laboratory of Molecular Pharmacology, Department of Systems Biomedicine, Research Center Kobe, Bayer Yakuhin Ltd, Kobe, Hyogo 650-0047, Japan

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Hiroshi Asahara Department of Stem Cell Based Drug Discovery, Division of Sex Differentiation, Laboratory of Molecular Pharmacology, Department of Systems Biomedicine, Research Center Kobe, Bayer Yakuhin Ltd, Kobe, Hyogo 650-0047, Japan

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Tetsuya Ishikawa
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Fumiki Shimada
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Kazuhiro Sakurada
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regulation of aromatase in developing ovary and placenta . Journal of Steroid Biochemistry and Molecular Biology 106 62 – 70 . Minguell JJ Erices A Conget P 2001 Mesenchymal stem cells . Experimental Biology and Medicine 226 507 – 520

Article Type:
Research Article
DOI:
https://doi.org/10.1677/JOE-08-0027
Volume/Issue:
Volume 198: Issue 3
Free access
Jin-Ran Chen Arkansas Children’s Nutrition Center, Little Rock, Arkansas, USA
Department of Pediatrics, University of Arkansas for Medical Sciences, Little Rock, Arkansas, USA

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Oxana P Lazarenko Arkansas Children’s Nutrition Center, Little Rock, Arkansas, USA
Department of Pediatrics, University of Arkansas for Medical Sciences, Little Rock, Arkansas, USA

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Haijun Zhao Arkansas Children’s Nutrition Center, Little Rock, Arkansas, USA
Department of Pediatrics, University of Arkansas for Medical Sciences, Little Rock, Arkansas, USA

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Alexander W Alund Interdisciplinary Biomedical Sciences , University of Arkansas for Medical Sciences, Little Rock, Arkansas, USA

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Kartik Shankar Arkansas Children’s Nutrition Center, Little Rock, Arkansas, USA
Department of Pediatrics, University of Arkansas for Medical Sciences, Little Rock, Arkansas, USA

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and p21 in UC MSCs from obese mothers compared to those cells from control lean mothers ( Fig. 4B ). Figure 3 Increased senescence signaling in mesenchymal stem cells (MSCs) from umbilical cords (UC) of obese mothers. (A) Human UC MSCs either

Article Type:
Research Article
DOI:
https://doi.org/10.1530/JOE-18-0244
Volume/Issue:
Volume 239: Issue 1
Open access
S Benvenuti
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P Luciani
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I Cellai
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C Deledda
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S Baglioni
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R Saccardi Endocrine Unit, Department of Haematology, Anatomy, Section of Endocrinology, Department of Clinical Physiopathology, Center for Research, Transfer and High Education on Chronic, Inflammatory, Degenerative and Neoplastic Disorders for the Development of Novel Therapies’ (DENOThe), University of Florence, Viale Pieraccini, 6, 50139 Florence, Italy

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S Urbani Endocrine Unit, Department of Haematology, Anatomy, Section of Endocrinology, Department of Clinical Physiopathology, Center for Research, Transfer and High Education on Chronic, Inflammatory, Degenerative and Neoplastic Disorders for the Development of Novel Therapies’ (DENOThe), University of Florence, Viale Pieraccini, 6, 50139 Florence, Italy

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F Francini Endocrine Unit, Department of Haematology, Anatomy, Section of Endocrinology, Department of Clinical Physiopathology, Center for Research, Transfer and High Education on Chronic, Inflammatory, Degenerative and Neoplastic Disorders for the Development of Novel Therapies’ (DENOThe), University of Florence, Viale Pieraccini, 6, 50139 Florence, Italy

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R Squecco Endocrine Unit, Department of Haematology, Anatomy, Section of Endocrinology, Department of Clinical Physiopathology, Center for Research, Transfer and High Education on Chronic, Inflammatory, Degenerative and Neoplastic Disorders for the Development of Novel Therapies’ (DENOThe), University of Florence, Viale Pieraccini, 6, 50139 Florence, Italy

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C Giuliani
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G B Vannelli Endocrine Unit, Department of Haematology, Anatomy, Section of Endocrinology, Department of Clinical Physiopathology, Center for Research, Transfer and High Education on Chronic, Inflammatory, Degenerative and Neoplastic Disorders for the Development of Novel Therapies’ (DENOThe), University of Florence, Viale Pieraccini, 6, 50139 Florence, Italy

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M Serio
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A Pinchera Endocrine Unit, Department of Haematology, Anatomy, Section of Endocrinology, Department of Clinical Physiopathology, Center for Research, Transfer and High Education on Chronic, Inflammatory, Degenerative and Neoplastic Disorders for the Development of Novel Therapies’ (DENOThe), University of Florence, Viale Pieraccini, 6, 50139 Florence, Italy

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A Peri
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demonstrated that high levels of expression of seladin-1 were detectable in human mesenchymal stem cells (hMSC), whereas a significant reduction was observed in hMSC-derived neurons (hMSC-n; Benvenuti et al . 2006 ). We have also detected high levels of

Article Type:
Research Article
DOI:
https://doi.org/10.1677/JOE-07-0324
Volume/Issue:
Volume 197: Issue 2
Free access
Fan He School of Engineering, Orthopaedic Institute, Department of Orthopaedics, Nanfang Hospital, Stem Cell and Tissue Engineering Laboratory, Sun Yat-sen University, No. 132 East Waihuan Road, Guangzhou Higher Education Mega Center, Guangzhou 510006, China
School of Engineering, Orthopaedic Institute, Department of Orthopaedics, Nanfang Hospital, Stem Cell and Tissue Engineering Laboratory, Sun Yat-sen University, No. 132 East Waihuan Road, Guangzhou Higher Education Mega Center, Guangzhou 510006, China
School of Engineering, Orthopaedic Institute, Department of Orthopaedics, Nanfang Hospital, Stem Cell and Tissue Engineering Laboratory, Sun Yat-sen University, No. 132 East Waihuan Road, Guangzhou Higher Education Mega Center, Guangzhou 510006, China

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Xiaozhen Liu School of Engineering, Orthopaedic Institute, Department of Orthopaedics, Nanfang Hospital, Stem Cell and Tissue Engineering Laboratory, Sun Yat-sen University, No. 132 East Waihuan Road, Guangzhou Higher Education Mega Center, Guangzhou 510006, China

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Ke Xiong School of Engineering, Orthopaedic Institute, Department of Orthopaedics, Nanfang Hospital, Stem Cell and Tissue Engineering Laboratory, Sun Yat-sen University, No. 132 East Waihuan Road, Guangzhou Higher Education Mega Center, Guangzhou 510006, China

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Sijin Chen School of Engineering, Orthopaedic Institute, Department of Orthopaedics, Nanfang Hospital, Stem Cell and Tissue Engineering Laboratory, Sun Yat-sen University, No. 132 East Waihuan Road, Guangzhou Higher Education Mega Center, Guangzhou 510006, China

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Long Zhou School of Engineering, Orthopaedic Institute, Department of Orthopaedics, Nanfang Hospital, Stem Cell and Tissue Engineering Laboratory, Sun Yat-sen University, No. 132 East Waihuan Road, Guangzhou Higher Education Mega Center, Guangzhou 510006, China
School of Engineering, Orthopaedic Institute, Department of Orthopaedics, Nanfang Hospital, Stem Cell and Tissue Engineering Laboratory, Sun Yat-sen University, No. 132 East Waihuan Road, Guangzhou Higher Education Mega Center, Guangzhou 510006, China

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Wenguo Cui School of Engineering, Orthopaedic Institute, Department of Orthopaedics, Nanfang Hospital, Stem Cell and Tissue Engineering Laboratory, Sun Yat-sen University, No. 132 East Waihuan Road, Guangzhou Higher Education Mega Center, Guangzhou 510006, China
School of Engineering, Orthopaedic Institute, Department of Orthopaedics, Nanfang Hospital, Stem Cell and Tissue Engineering Laboratory, Sun Yat-sen University, No. 132 East Waihuan Road, Guangzhou Higher Education Mega Center, Guangzhou 510006, China

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Guoqing Pan School of Engineering, Orthopaedic Institute, Department of Orthopaedics, Nanfang Hospital, Stem Cell and Tissue Engineering Laboratory, Sun Yat-sen University, No. 132 East Waihuan Road, Guangzhou Higher Education Mega Center, Guangzhou 510006, China
School of Engineering, Orthopaedic Institute, Department of Orthopaedics, Nanfang Hospital, Stem Cell and Tissue Engineering Laboratory, Sun Yat-sen University, No. 132 East Waihuan Road, Guangzhou Higher Education Mega Center, Guangzhou 510006, China

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Zong-Ping Luo School of Engineering, Orthopaedic Institute, Department of Orthopaedics, Nanfang Hospital, Stem Cell and Tissue Engineering Laboratory, Sun Yat-sen University, No. 132 East Waihuan Road, Guangzhou Higher Education Mega Center, Guangzhou 510006, China
School of Engineering, Orthopaedic Institute, Department of Orthopaedics, Nanfang Hospital, Stem Cell and Tissue Engineering Laboratory, Sun Yat-sen University, No. 132 East Waihuan Road, Guangzhou Higher Education Mega Center, Guangzhou 510006, China

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Ming Pei School of Engineering, Orthopaedic Institute, Department of Orthopaedics, Nanfang Hospital, Stem Cell and Tissue Engineering Laboratory, Sun Yat-sen University, No. 132 East Waihuan Road, Guangzhou Higher Education Mega Center, Guangzhou 510006, China

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Yihong Gong School of Engineering, Orthopaedic Institute, Department of Orthopaedics, Nanfang Hospital, Stem Cell and Tissue Engineering Laboratory, Sun Yat-sen University, No. 132 East Waihuan Road, Guangzhou Higher Education Mega Center, Guangzhou 510006, China

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human mesenchymal stem cells . Journal of Pineal Research 55 14 – 25 . ( doi:10.1111/jpi.12045 ) Liu X Xu Y Chen S Tan Z Xiong K Li Y Ye Y Luo ZP He F Gong Y 2014 Rescue of proinflammatory cytokine-inhibited chondrogenesis by

Article Type:
Research Article
DOI:
https://doi.org/10.1530/JOE-14-0430
Volume/Issue:
Volume 223: Issue 2
Free access
Sujith Rajan Division of Pharmacology, CSIR-Central Drug Research Institute, Lucknow, India
Academy of Scientific and Innovative Research, CSIR-CDRI, Lucknow, India

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Ganesh Panzade Studio of Computational Biology and Bioinformatics, Biotechnology Division, CSIR-Institute of Himalayan Bioresource Technology, Palampur, India
Academy of Scientific and Innovative Research, CSIR-IHBT, Palampur, India

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Ankita Srivastava Division of Pharmacology, CSIR-Central Drug Research Institute, Lucknow, India
Academy of Scientific and Innovative Research, CSIR-CDRI, Lucknow, India

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Kripa Shankar Division of Pharmacology, CSIR-Central Drug Research Institute, Lucknow, India

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Rajesh Pandey CSIR Ayurgenomics Unit-TRISUTRA, CSIR-Institute of Genomics and Integrative Biology, New Delhi, India

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Durgesh Kumar Division of Pharmacology, CSIR-Central Drug Research Institute, Lucknow, India
Academy of Scientific and Innovative Research, CSIR-CDRI, Lucknow, India

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Sanchita Gupta Division of Pharmacology, CSIR-Central Drug Research Institute, Lucknow, India
Academy of Scientific and Innovative Research, CSIR-CDRI, Lucknow, India

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Abhishek Gupta Division of Pharmacology, CSIR-Central Drug Research Institute, Lucknow, India

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Salil Varshney Division of Pharmacology, CSIR-Central Drug Research Institute, Lucknow, India
Academy of Scientific and Innovative Research, CSIR-CDRI, Lucknow, India

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Muheeb Beg Division of Pharmacology, CSIR-Central Drug Research Institute, Lucknow, India

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Raj Kumar Mishra SIPS Superspeciality Hospital, Lucknow, India

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Ravi Shankar Studio of Computational Biology and Bioinformatics, Biotechnology Division, CSIR-Institute of Himalayan Bioresource Technology, Palampur, India
Academy of Scientific and Innovative Research, CSIR-IHBT, Palampur, India

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Anil Gaikwad Division of Pharmacology, CSIR-Central Drug Research Institute, Lucknow, India
Academy of Scientific and Innovative Research, CSIR-CDRI, Lucknow, India

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diabetes. Materials and methods hMSC culture and differentiation into white and brown adipocytes Human mesenchymal stem cell (hMSC) was cultured in high-glucose DMEM supplemented with 10% fetal bovine serum (Gibco) and 1% antibiotics. hMSC was

Article Type:
Research Article
DOI:
https://doi.org/10.1530/JOE-17-0387
Volume/Issue:
Volume 239: Issue 1
Free access
CN Street
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Lakey JR
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K Seeberger
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L Helms
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RV Rajotte
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AM Shapiro
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GS Korbutt
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The discovery of a pancreatic adult stem cell would have significant implications for cell-based replacement therapies for type 1 diabetes mellitus. Nestin, a marker for neural precursor cells, has been suggested as a possible marker for islet progenitor cells. We have characterized the expression and localization of nestin in both the intact human pancreas and clinical human pancreatic islet grafts. Nestin was found to be expressed at different levels in the acinar component of human pancreatic biopsies depending on donor, as well as in ductal structures and islets to some degree. In islets, insulin-producing beta-cells rarely co-expressed the protein, and in the ducts a small percentage (1-2%) of cells co-expressed nestin and cytokeratin 19 (CK19) while most expressed only CK19 (90%) or nestin (5-10%) alone. Assessment of nestin expression in neonatal pancreatic sections revealed an increased number of islet-associated positive cells as compared with adult islets. Nestin immunoreactivity was also found in cells of the pancreatic vasculature and mesenchyme as evidenced by co-localization with smooth muscle actin and vimentin. Samples from post-islet isolation clinical islet grafts revealed a pronounced heterogeneity in the proportion of nestin-positive cells (<1-72%). Co-localization studies in these grafts showed that nestin is not co-expressed in endocrine cells and rarely (<5%) with cytokeratin-positive ductal cells. However, relatively high levels of co-expression were found with acinar cells and cells expressing the mesenchymal marker vimentin. In conclusion we have shown a diffuse and variable expression of nestin in human pancreas that may be due to a number of different processes, including post-mortem tissue remodeling and cellular differentiation. For this reason nestin may not be a suitable marker solely for the identification of endocrine precursor cells in the pancreas.

Article Type:
Other
DOI:
https://doi.org/10.1677/joe.0.1800213
Volume/Issue:
Volume 180: Issue 2
Free access
Mugdha V Joglekar
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Vinay M Joglekar Stem Cells and Diabetes Section, Shree Seva Medical Foundation, Lab 10, National Center for Cell Science, Ganeshkhind Road, Pune MH 411007, India

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Sheela V Joglekar Stem Cells and Diabetes Section, Shree Seva Medical Foundation, Lab 10, National Center for Cell Science, Ganeshkhind Road, Pune MH 411007, India

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Anandwardhan A Hardikar
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. Mutskov VRB Felsenfeld G Gershengorn MC 2007 The human insulin gene displays transcriptionally active epigenetic marks in islet-derived mesenchymal precursor cells in the absence of insulin expression . Stem Cells 25 3223 – 3233 . Rankin

Article Type:
Research Article
DOI:
https://doi.org/10.1677/JOE-08-0497
Volume/Issue:
Volume 201: Issue 1
Free access
Min Zhao
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Stephanie A Amiel
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Michael R Christie
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Paolo Muiesan Department of Diabetes, Institutes of Liver Studies, School of Biomedical and Health Sciences, Endocrinology and Internal Medicine, King's College London School of Medicine, Bessemer Road, London SE5 9PJ, UK

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Parthi Srinivasan Department of Diabetes, Institutes of Liver Studies, School of Biomedical and Health Sciences, Endocrinology and Internal Medicine, King's College London School of Medicine, Bessemer Road, London SE5 9PJ, UK

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Wendy Littlejohn Department of Diabetes, Institutes of Liver Studies, School of Biomedical and Health Sciences, Endocrinology and Internal Medicine, King's College London School of Medicine, Bessemer Road, London SE5 9PJ, UK

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Mohamed Rela Department of Diabetes, Institutes of Liver Studies, School of Biomedical and Health Sciences, Endocrinology and Internal Medicine, King's College London School of Medicine, Bessemer Road, London SE5 9PJ, UK

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Matthew Arno Department of Diabetes, Institutes of Liver Studies, School of Biomedical and Health Sciences, Endocrinology and Internal Medicine, King's College London School of Medicine, Bessemer Road, London SE5 9PJ, UK

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Nigel Heaton Department of Diabetes, Institutes of Liver Studies, School of Biomedical and Health Sciences, Endocrinology and Internal Medicine, King's College London School of Medicine, Bessemer Road, London SE5 9PJ, UK

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Guo Cai Huang
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the progression of Type 2 diabetes. The presence of adult stem cells or pancreatic-specific progenitor cells in human pancreas is controversial. In animals, the β-cell population does increase following pancreas duct ligation ( Wang et al . 1995 ) or

Article Type:
Research Article
DOI:
https://doi.org/10.1677/JOE-07-0436
Volume/Issue:
Volume 195: Issue 3
Free access