, recombinant adeno-associated viral techniques were employed to target leptin overexpression in the VTA or NTS coupled with the simultaneous overexpression of a dominant negative leptin mutant (leptin antagonist) in the MBH. Food intake and body weight (BW
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M Matheny, K Y E Strehler, M King, N Tümer, and P J Scarpace
I Sadaf Farooqi and Stephen O'Rahilly
to leptin administration in leptin deficiency Although leptin deficiency is rare, it is entirely treatable with daily s.c. injections of recombinant human leptin ( Farooqi et al . 1999 , Licinio et al . 2004 ). Such treatment is currently available
Alex M DePaoli
of leptin. Recombinant leptin has also been studied in patients with T2D to assess both weight loss and metabolic effects independent of weight loss. These studies have underscored the challenges of defining effective therapeutic applications of
Gideon Hen, Sera Yosefi, Ana Ronin, Paz Einat, Charles I Rosenblum, Robert J Denver, and Miriam Friedman-Einat
describe the construction of a leptin bioassay based on the forced expression in cultured cells of CLEPR. We show that recombinant leptins originating from both humans and frogs activate CLEPR with high potency. This bioassay is expected to be valuable for
Marcela Oliveira, Diego M Assis, Thaysa Paschoalin, Antonio Miranda, Eliane B Ribeiro, Maria A Juliano, Dieter Brömme, Marcelo Augusto Christoffolete, Nilana M T Barros, and Adriana K Carmona
a BCA protein assay (Pierce Biotechnology, Inc., Rockford, IL, USA) with BSA as a standard. Proteins Recombinant human leptin was purchased from R&D (Minneapolis, MN, USA). The recombinant human cathepsins S and K were expressed in Pichia pastoris
Russell T Turner, Michael Dube, Adam J Branscum, Carmen P Wong, Dawn A Olson, Xiaoying Zhong, Mercedes F Kweh, Iske V Larkin, Thomas J Wronski, Clifford J Rosen, Satya P Kalra, and Urszula T Iwaniec
. Journal of Bone and Mineral Research 25 2078 – 2088 . ( doi:10.1002/jbmr.82 ) Dhillon H. 2000 Effects of recombinant adeno-associated virus encoding leptin on body weight regulation and energy homeostasis. p 171: University of Florida. Dhillon H Ge
Courtney A Deck, Jamie L Mankiewicz, and Russell J Borski
shaker at 60 rpm for 2 h. After this pre-incubation period, the media was removed from each well and replaced with 100 µL of HBSS containing 1, 10, or 50 nM recombinant tilapia leptin A (rtLepA; n = 5 wells/treatment). Control wells were refilled with
Kenneth A Philbrick, Stephen A Martin, Amy R Colagiovanni, Adam J Branscum, Russell T Turner, and Urszula T Iwaniec
growing (4-month-old) and skeletally mature (6-month-old) WT and ob/ob male mice. To address the second question, we employed hypothalamic recombinant adeno-associated virus (rAAV) gene therapy in 2-month-old ob/ob mice to restore leptin signaling and
M G Gnanalingham, A Mostyn, J Wang, R Webb, D H Keisler, N Raver, M C Alves-Guerra, C Pecqueur, B Miroux, T Stephenson, and M E Symonds
mRNA are enhanced by recombinant adenoviral-induced leptin expression ( Zhou et al. 1997 ) and UCP2 has also been genetically linked to type II diabetes ( Fleury et al. 1997 ). In the brain, UCP2 is expressed discretely in neurons located in the
Qingling Huang, Elena Timofeeva, and Denis Richard
(Model 2001; ALZA Pharmaceuticals, Palo Alto, CA, USA; 1.0 μl/h), which contained PBS (pH 7.4) or recombinant murine leptin (2 μg/day per rat; high purity, endotoxin level < 0.1 ng/μg; US Biological, Swampscott, MA, USA). PBS or leptin was continuously
