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A Margot Umpleby Diabetes and Metabolic Medicine, Faculty of Health and Metabolic Sciences, University of Surrey, Leggett Building, Daphne Jackson Road, Manor Park, Guildford GU2 7WG, UK

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. When one of these less abundant isotopes is substituted for the common form in a molecule, this is known as ‘labelling’ and creates a ‘tracer’. The original molecule is known as the tracee. The tracer and tracee have the same chemical properties. The

Article Type:
Research Article
DOI:
https://doi.org/10.1530/JOE-14-0610
Volume/Issue:
Volume 226: Issue 3
Free access
J. J. EVANS
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FELICITY J. WHITE
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IRIS L. SIN
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A single-point progesterone tracer binding assay (TBA) was developed to measure the concentration of progesterone-binding globulin (PBG) in the serum of pregnant guinea-pigs. Tritiated progesterone was added to dilutions of a reference serum, the percentage bound calculated and a standard curve constructed. The amount of progesterone tracer bound to serum samples was determined, and the concentration of binding protein in the sample relative to the reference was calculated. The molar concentration in the reference serum was determined by Scatchard analysis. The TBA could readily process 30 samples per day. There was a rapid rise in PBG concentrations between days 15 and 20 of gestation, in parallel with progesterone concentrations. The molar ratio of PBG: progesterone was approximately 10: 1 after this time, until about day 45 when there was an increase in ratio.

Article Type:
Research Article
DOI:
https://doi.org/10.1677/joe.0.0900331
Volume/Issue:
Volume 90: Issue 3
Restricted access
Keld Fosgerau In vivoPharmacology, Rheoscience, Ledøje Bygade 23B, DK-2765 Ledøje-Smørum, Denmark
Novo Nordisk Discovery and Development, Novo Nordisk A/S, Novo Nordisk Park, DK-2760 Maaloev, Denmark
Garvan Institute of Medical Research, 384 Victoria Street, Sydney NSW 2010, Australia

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Christian Fledelius In vivoPharmacology, Rheoscience, Ledøje Bygade 23B, DK-2765 Ledøje-Smørum, Denmark
Novo Nordisk Discovery and Development, Novo Nordisk A/S, Novo Nordisk Park, DK-2760 Maaloev, Denmark
Garvan Institute of Medical Research, 384 Victoria Street, Sydney NSW 2010, Australia

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Kent E Pedersen In vivoPharmacology, Rheoscience, Ledøje Bygade 23B, DK-2765 Ledøje-Smørum, Denmark
Novo Nordisk Discovery and Development, Novo Nordisk A/S, Novo Nordisk Park, DK-2760 Maaloev, Denmark
Garvan Institute of Medical Research, 384 Victoria Street, Sydney NSW 2010, Australia

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Jesper B Kristensen In vivoPharmacology, Rheoscience, Ledøje Bygade 23B, DK-2765 Ledøje-Smørum, Denmark
Novo Nordisk Discovery and Development, Novo Nordisk A/S, Novo Nordisk Park, DK-2760 Maaloev, Denmark
Garvan Institute of Medical Research, 384 Victoria Street, Sydney NSW 2010, Australia

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Jens R Daugaard In vivoPharmacology, Rheoscience, Ledøje Bygade 23B, DK-2765 Ledøje-Smørum, Denmark
Novo Nordisk Discovery and Development, Novo Nordisk A/S, Novo Nordisk Park, DK-2760 Maaloev, Denmark
Garvan Institute of Medical Research, 384 Victoria Street, Sydney NSW 2010, Australia

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Miguel A Iglesias In vivoPharmacology, Rheoscience, Ledøje Bygade 23B, DK-2765 Ledøje-Smørum, Denmark
Novo Nordisk Discovery and Development, Novo Nordisk A/S, Novo Nordisk Park, DK-2760 Maaloev, Denmark
Garvan Institute of Medical Research, 384 Victoria Street, Sydney NSW 2010, Australia

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Edward W Kraegen In vivoPharmacology, Rheoscience, Ledøje Bygade 23B, DK-2765 Ledøje-Smørum, Denmark
Novo Nordisk Discovery and Development, Novo Nordisk A/S, Novo Nordisk Park, DK-2760 Maaloev, Denmark
Garvan Institute of Medical Research, 384 Victoria Street, Sydney NSW 2010, Australia

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Stuart M Furler In vivoPharmacology, Rheoscience, Ledøje Bygade 23B, DK-2765 Ledøje-Smørum, Denmark
Novo Nordisk Discovery and Development, Novo Nordisk A/S, Novo Nordisk Park, DK-2760 Maaloev, Denmark
Garvan Institute of Medical Research, 384 Victoria Street, Sydney NSW 2010, Australia

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-prandial absorptive phase. Our experiments were performed in rats, using the bromopalmitate/palmitate double tracer technique ( Oakes et al. 1999 ), which involves simultaneous administration of the partly metabolizable FFA tracer [9,10- 3 H]-( R )-2-bromopalmitate

Article Type:
Research Article
DOI:
https://doi.org/10.1677/joe.1.06809
Volume/Issue:
Volume 190: Issue 3
Free access
T’ng Choong Kwok University/BHF Centre for Cardiovascular Science, University of Edinburgh, Queen’s Medical Research Institute, Edinburgh, United Kingdom

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Roland H Stimson University/BHF Centre for Cardiovascular Science, University of Edinburgh, Queen’s Medical Research Institute, Edinburgh, United Kingdom

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, Koskensalo et al. 2017 ). Additionally, BAT volume quantified with 18 F-FDG has not been substantially different to those visualised using other tracers ( Admiraal et al. 2013 ). Early studies analysing clinical 18 F-FDG PET/CT scans undertaken at

Article Type:
Review Article
DOI:
https://doi.org/10.1530/JOE-23-0017
Volume/Issue:
Volume 259: Issue 1
Open access
Piotr Zabielski Department of Medical Biology, Medical University of Bialystok, Bialystok, Poland
Department of Physiology, Medical University of Bialystok, Bialystok, Poland

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Marta Chacinska Department of Physiology, Medical University of Bialystok, Bialystok, Poland
Department of Hygiene, Epidemiology and Metabolic Disorders, Medical University of Bialystok, Bialystok, Poland

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Karol Charkiewicz Department of Physiology, Medical University of Bialystok, Bialystok, Poland
Department of Perinatology, Medical University of Bialystok, Bialystok, Poland

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Marcin Baranowski Department of Physiology, Medical University of Bialystok, Bialystok, Poland

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Jan Gorski Department of Physiology, Medical University of Bialystok, Bialystok, Poland

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Agnieszka U Blachnio-Zabielska Department of Physiology, Medical University of Bialystok, Bialystok, Poland
Department of Hygiene, Epidemiology and Metabolic Disorders, Medical University of Bialystok, Bialystok, Poland

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or release from complex sphingolipids such as sphingomyelins, glucosyl and galactosyl ceramides. Conversely, reduction in intramuscular Cer can arise from its hydrolysis or incorporation into complex sphingolipids. The use of stable isotope tracers

Article Type:
Research Article
DOI:
https://doi.org/10.1530/JOE-16-0381
Volume/Issue:
Volume 233: Issue 3
Free access
Hidenori Nagao Pharmacokinetics Research Department of ASKA Pharmaceutical Co., Synthetic Research Department of ASKA Pharmaceutical Co., ASKA Pharma Medical Co., Ltd

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Tetsuya Imazu Pharmacokinetics Research Department of ASKA Pharmaceutical Co., Synthetic Research Department of ASKA Pharmaceutical Co., ASKA Pharma Medical Co., Ltd

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Hiroyuki Hayashi Pharmacokinetics Research Department of ASKA Pharmaceutical Co., Synthetic Research Department of ASKA Pharmaceutical Co., ASKA Pharma Medical Co., Ltd

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Kenjo Takahashi Pharmacokinetics Research Department of ASKA Pharmaceutical Co., Synthetic Research Department of ASKA Pharmaceutical Co., ASKA Pharma Medical Co., Ltd

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Kouichi Minato Pharmacokinetics Research Department of ASKA Pharmaceutical Co., Synthetic Research Department of ASKA Pharmaceutical Co., ASKA Pharma Medical Co., Ltd

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specimens. One of the unique advantages of this method, which uses a stable isotope-labeled compound as a tracer, is that an endogenous compound and its exogenously administered labeled analog can be measured separately using LC–MS/MS ( Lin et al . 2010

Article Type:
Research Article
DOI:
https://doi.org/10.1530/JOE-10-0484
Volume/Issue:
Volume 210: Issue 1
Free access
Li Zhao Institute and Department of Endocrinology and Metabolism, Shanghai Ninth People’s Hospital, Shanghai JiaoTong University School of Medicine, Shanghai, China

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Chunfang Zhu Institute and Department of Endocrinology and Metabolism, Shanghai Ninth People’s Hospital, Shanghai JiaoTong University School of Medicine, Shanghai, China

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Meng Lu Research Center for Clinical Medicine, Shanghai Ninth People’s Hospital, Shanghai JiaoTong University School of Medicine, Shanghai, China

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Chi Chen Institute and Department of Endocrinology and Metabolism, Shanghai Ninth People’s Hospital, Shanghai JiaoTong University School of Medicine, Shanghai, China

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Xiaomin Nie Institute and Department of Endocrinology and Metabolism, Shanghai Ninth People’s Hospital, Shanghai JiaoTong University School of Medicine, Shanghai, China

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Buatikamu Abudukerimu Institute and Department of Endocrinology and Metabolism, Shanghai Ninth People’s Hospital, Shanghai JiaoTong University School of Medicine, Shanghai, China

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Kun Zhang Institute and Department of Endocrinology and Metabolism, Shanghai Ninth People’s Hospital, Shanghai JiaoTong University School of Medicine, Shanghai, China

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Zhiyuan Ning Institute and Department of Endocrinology and Metabolism, Shanghai Ninth People’s Hospital, Shanghai JiaoTong University School of Medicine, Shanghai, China

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Yi Chen Institute and Department of Endocrinology and Metabolism, Shanghai Ninth People’s Hospital, Shanghai JiaoTong University School of Medicine, Shanghai, China

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Jing Cheng Institute and Department of Endocrinology and Metabolism, Shanghai Ninth People’s Hospital, Shanghai JiaoTong University School of Medicine, Shanghai, China

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Fangzhen Xia Institute and Department of Endocrinology and Metabolism, Shanghai Ninth People’s Hospital, Shanghai JiaoTong University School of Medicine, Shanghai, China

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Ningjian Wang Institute and Department of Endocrinology and Metabolism, Shanghai Ninth People’s Hospital, Shanghai JiaoTong University School of Medicine, Shanghai, China

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Michael D Jensen Endocrine Research Unit, Mayo Clinic, Rochester, Minnesota, USA

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Yingli Lu Institute and Department of Endocrinology and Metabolism, Shanghai Ninth People’s Hospital, Shanghai JiaoTong University School of Medicine, Shanghai, China

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beginning when their food was fully consumed. After local anesthesia with lidocaine, the lateral tail vein was catheterized for the infusion of tracers, and the tail artery was catheterized for blood sampling using previously described methods ( Guo & Zhou

Article Type:
Research Article
DOI:
https://doi.org/10.1530/JOE-18-0374
Volume/Issue:
Volume 240: Issue 2
Free access
Gabriel O de Souza Departamento de Fisiologia e Biofisica, Instituto de Ciencias Biomedicas, Universidade de Sao Paulo, Sao Paulo, Sao Paulo, Brazil

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Fernanda M Chaves Departamento de Fisiologia e Biofisica, Instituto de Ciencias Biomedicas, Universidade de Sao Paulo, Sao Paulo, Sao Paulo, Brazil

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Josiane N Silva Departamento de Anatomia, Instituto de Ciencias Biomedicas, Universidade de Sao Paulo, Sao Paulo, Sao Paulo, Brazil

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João A B Pedroso Departamento de Fisiologia e Biofisica, Instituto de Ciencias Biomedicas, Universidade de Sao Paulo, Sao Paulo, Sao Paulo, Brazil

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Martin Metzger Departamento de Fisiologia e Biofisica, Instituto de Ciencias Biomedicas, Universidade de Sao Paulo, Sao Paulo, Sao Paulo, Brazil

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Renata Frazão Departamento de Anatomia, Instituto de Ciencias Biomedicas, Universidade de Sao Paulo, Sao Paulo, Sao Paulo, Brazil

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Jose Donato Departamento de Fisiologia e Biofisica, Instituto de Ciencias Biomedicas, Universidade de Sao Paulo, Sao Paulo, Sao Paulo, Brazil

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subset of AgRP cells ( n  = 6), the recordings were performed with a biocytin-filled pipette (3%; Sigma-Aldrich) to detect the possible spread of this tracer to surrounding cells. The membrane potential was monitored for at least 5 min (Basal), followed

Article Type:
Research Article
DOI:
https://doi.org/10.1530/JOE-22-0012
Volume/Issue:
Volume 255: Issue 2
Restricted access
Munetaka Shimizu Northwest Fisheries Science Center, NOAA Fisheries, 2725 Montlake Boulevard East, Seattle, Washington 98112, USA
School of Aquatic and Fishery Sciences, University of Washington, Seattle, Washington 98195, USA
Graduate School of Fisheries Sciences, Hokkaido University, 3-1–1 Minato, Hakodate, Hokkaido 041-8611, Japan

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Brian R Beckman Northwest Fisheries Science Center, NOAA Fisheries, 2725 Montlake Boulevard East, Seattle, Washington 98112, USA
School of Aquatic and Fishery Sciences, University of Washington, Seattle, Washington 98195, USA
Graduate School of Fisheries Sciences, Hokkaido University, 3-1–1 Minato, Hakodate, Hokkaido 041-8611, Japan

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Akihiko Hara Northwest Fisheries Science Center, NOAA Fisheries, 2725 Montlake Boulevard East, Seattle, Washington 98112, USA
School of Aquatic and Fishery Sciences, University of Washington, Seattle, Washington 98195, USA
Graduate School of Fisheries Sciences, Hokkaido University, 3-1–1 Minato, Hakodate, Hokkaido 041-8611, Japan

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Walton W Dickhoff Northwest Fisheries Science Center, NOAA Fisheries, 2725 Montlake Boulevard East, Seattle, Washington 98112, USA
School of Aquatic and Fishery Sciences, University of Washington, Seattle, Washington 98195, USA
Graduate School of Fisheries Sciences, Hokkaido University, 3-1–1 Minato, Hakodate, Hokkaido 041-8611, Japan

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). Sample analyses Plasma IGF-I levels were measured by RIA as described in Shimizu et al. (2000) . Briefly, IGF-I was first extracted from plasma by acid-ethanol and quantified by the RIA using recombinant salmon IGF-I as standard and tracer

Article Type:
Research Article
DOI:
https://doi.org/10.1677/joe.1.06475
Volume/Issue:
Volume 188: Issue 1
Free access
Edward Park
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Victor Wong
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Xinyu Guan
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Andrei I Oprescu
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Adria Giacca
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–euglycemic clamp was performed with tracer infusion during the last 2 h of the 7-h infusion period to assess hepatic and peripheral insulin sensitivity. During 30 min preceding the clamp (‘basal period’), measurements were taken at 10-min interval for plasma

Article Type:
Research Article
DOI:
https://doi.org/10.1677/JOE-07-0005
Volume/Issue:
Volume 195: Issue 2
Free access