Expression of the parathyroid Ca(2+)-sensing receptor in cytotrophoblasts from human term placenta

in Journal of Endocrinology
Authors:
RA Bradbury
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KL Sunn
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M Crossley
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M Bai
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EM Brown
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L Delbridge
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, and
AD Conigrave
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DOI:
https://doi.org/10.1677/joe.0.1560425
Volume/Issue:
Volume 156: Issue 3
Article Type:
Other
Page Range:
425–430
Online Publication Date:
01 Mar 1998
Free access

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Fura-2-loaded human cytotrophoblasts responded to elevated extracellular Ca2+ concentration ([Ca2+]o) with monophasic or, in the case of large (> 20 microns) extravillous cells, biphasic elevations in intracellular free Ca2+ ion concentration ([Ca2+]i) that returned to baseline levels after restoration of control [Ca2+]o. Large extravillous cytotrophoblasts also responded to elevated [Mg2+]o with transient elevations in [Ca2+]i, consistent with the behaviour of the parathyroid Ca2(+)-sensing receptor. Expression of the parathyroid Ca2(+)-sensing receptor in placental cells was confirmed using Northern blot and reverse transcription (RT)-PCR analysis. However, the major transcript in human placental cells (6.2 kb) differed from that expressed by human parathyroid cells (5.6 kb). RT-PCR analysis and DNA sequencing of key PCR products also revealed the presence of a splice variant in placental and parathyroid cells that lacks exon 3.

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Print ISSN:
0022-0795
Online ISSN:
1479-6805
Page(s):
6
Article Type:
Other
Print Publication Date:
01 Mar 1998
Online Publication Date:
01 Mar 1998