Effects of activin on hormone secretion by single female rat pituitary cells: analysis by cell immunoblot assay

in Journal of Endocrinology
Authors:
S Miyamoto
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,
M Irahara
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K Ushigoe
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A Kuwahara
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H Sugino
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T Aono
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DOI:
https://doi.org/10.1677/joe.0.1610375
Volume/Issue:
Volume 161: Issue 3
Article Type:
Other
Page Range:
375–382
Online Publication Date:
01 Jun 1999
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We investigated the effect of activin A on secretion of LH, FSH, and prolactin (PRL) by female cultured rat pituitary cells at the single-cell level by means of the cell immunoblot assay. Anterior pituitary cells from 8-week-old female rats were preincubated with or without activin A for 24 h, after which they were monodispersed and immediately used for cell immunoblot assay. The percentages of LH-, FSH- and PRL-immunoreactive cell blots detected were 5.5, 5.3 and 43.1%, respectively, of all pituitary cells applied to the transfer membrane. The percentage of LH-secreting cells and mean LH secretion per cell did not change after treatment with activin. In contrast, activin significantly increased the percentage of FSH-secreting cells and mean FSH secretion per cell to 136.0 and 114. 5% respectively. In addition, activin significantly decreased the percentage of PRL-secreting cells and mean PRL secretion per cell to 52.2 and 72.0% respectively. These results suggest that (1) activin A has effects on female rat pituitary cells that increase not only the amount of FSH secretion per cell but also the number of FSH-secreting cells, and (2) activin A decreases both the amount of PRL secretion per cell and the number of PRL-secreting cells.

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Print ISSN:
0022-0795
Online ISSN:
1479-6805
Page(s):
8
Article Type:
Other
Print Publication Date:
01 Jun 1999
Online Publication Date:
01 Jun 1999