NCX1 Na/Ca exchanger splice variants in pancreatic islet cells

in Journal of Endocrinology
Authors:
F Van Eylen
Search for other papers by F Van Eylen in
Current site
Google Scholar
PubMed Close
,
A Bollen
Search for other papers by A Bollen in
Current site
Google Scholar
PubMed Close
, and
A Herchuelz
Search for other papers by A Herchuelz in
Current site
Google Scholar
PubMed Close
DOI:
https://doi.org/10.1677/joe.0.1680517
Volume/Issue:
Volume 168: Issue 3
Article Type:
Other
Page Range:
517–526
Online Publication Date:
01 Mar 2001
Free access

Sign up for journal news

In the rat pancreatic beta-cell, Na/Ca exchange displays a quite high capacity. The cell is equipped with two alternatively spliced Na/Ca exchanger-1 (NCX1) isoforms, namely NCX1.3 and NCX1.7. To examine the existence of a possible functional difference between these splice variants, they were cloned, together with the heart variant NCX1.1, and expressed in human embryonic kidney-293 (HEK293) and Chinese hamster ovary (CHO) cells. In these two systems, the three splice variants showed a comparable level of intracellular Na+ (Na+(i))-dependent extracellular Ca2+(Ca2+(o)) uptake. Different levels of NCX1.3 and NCX1.7 transcripts were found in four rodent species, together with a marked interspecies difference in Na/Ca exchange activity. Three additional splice variants were found (NCX1.2, NCX1.9 and NCX1.13) in guinea-pigs, hamsters and mice, again in different proportions. Our data provide evidence for the activity of the three NCX1 splice variants. They also show the existence of a differential and species-specific transcription pattern of NCX1 gene products in pancreatic islet cells.

Journal of Endocrinology is committed to supporting researchers in demonstrating the impact of their articles published in the journal.

The two types of article metrics we measure are (i) more traditional full-text views and pdf downloads, and (ii) Altmetric data, which shows the wider impact of articles in a range of non-traditional sources, such as social media.

More information is on the Reasons to publish page.

Sept 2018 onwards Past Year Past 30 Days
Full Text Views 416 66 10
PDF Downloads 150 56 6

 

  • Collapse
  • Expand
Print ISSN:
0022-0795
Online ISSN:
1479-6805
Page(s):
10
Article Type:
Other
Print Publication Date:
01 Mar 2001
Online Publication Date:
01 Mar 2001