Angiotensin II stimulation of Na+/K+ATPase activity and cell growth by calcium-independent pathway in MCF-7 breast cancer cells

in Journal of Endocrinology
Authors:
A Muscella
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S Greco
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MG Elia
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C Storelli
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S Marsigliante
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DOI:
https://doi.org/10.1677/joe.0.1730315
Volume/Issue:
Volume 173: Issue 2
Article Type:
Other
Page Range:
315–323
Online Publication Date:
01 May 2002
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Here we demonstrated, by RT-PCR analysis, the expression of both angiotensin II (Ang II) receptor subtypes, AT1 and AT2, in a breast cancer epithelial cell line, MCF-7. Ang II was not able to affect the intracellular Ca2+ concentration in Fura-2 loaded cells suggesting that AT1-mediated phospholipid hydrolysis is not involved in its intracellular transduction pathway. Ang II modulated the activity of the Na+/K+ATPase in a dose- and time-dependent manner and was mitogenic, with a dose-dependent (1-1000 nM) proliferative effect and a maximal response at 100 nM. Both Na+/K+ATPase activation and stimulation of proliferation were mediated by binding of Ang II to AT1, as the effects were completely blocked by DuP 753, a specific AT1 antagonist. CGP 42112, an AT2 antagonist, did not affect Ang II actions. The main conclusion of this study is that Ang II exerts its effects on cell proliferation and Na+/K+ATPase in breast cancer epithelial cells, MCF-7, via AT1 activation independently of the Ca(2+) signalling mechanism.

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Print ISSN:
0022-0795
Online ISSN:
1479-6805
Page(s):
9
Article Type:
Other
Print Publication Date:
01 May 2002
Online Publication Date:
01 May 2002