Metabolism of thyroid hormones in cultured cardiac fibroblasts of neonatal rats

in Journal of Endocrinology
Authors:
SM van der Heide
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,
TJ Visser
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ME Everts
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PH Klaren
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DOI:
https://doi.org/10.1677/joe.0.1740111
Volume/Issue:
Volume 174: Issue 1
Article Type:
Other
Page Range:
111–119
Online Publication Date:
01 Jul 2002
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We have investigated the potential role of fibroblasts in local thyroid hormone metabolism in neonatal rat heart. Incubation of cardiac fibroblasts with thyroxine (T4) or 3,5,3'-tri-iodothyronine (T3) resulted in the appearance of water-soluble metabolites, whereas incubation of cardiomyocytes under the same conditions did not or did so to a much lesser extent. Time-course studies showed that production is already evident after 1-5 h of exposure and that the process equilibrates after 24-48 h. Analysis of the products revealed both the T4 and the T3 metabolites to be glucuronides. These results were corroborated by the detection of uridine diphosphate (UDP)-glucuronyltransferase activity in cardiac fibroblasts. We found no indication for outer ring deiodination in fibroblasts, cardiomyocytes or heart homogenates. From these results we have concluded that cardiac fibroblasts, but not cardiomyocytes, are able to glucuronidate T4 and T3 and secrete the conjugates. This could play a role in local metabolism, e.g. to protect the heart tissue from high levels of thyroid hormones.

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Print ISSN:
0022-0795
Online ISSN:
1479-6805
Page(s):
9
Article Type:
Other
Print Publication Date:
01 Jul 2002
Online Publication Date:
01 Jul 2002